Abstract
The present work shows that semistarvation (8–10 g of food for 10 days) increases netd-glucose, Na+ and water transport in the everted and perfused rat jejunum. A lincar and positive correlation between cell sugar concentration and transport was found in control and semistarved rats, but the phenomenon was more relevant only in semistarved animals. Membrane vesicle experiments showed that semistarvation increases sugar overshoot only in brush border membrane vesicles, while this situation does not occur in basolateral membrane vesicles. The effect of partial food deprivation seems to enhance net sugar transport by mereasing sugar entry across the apical membrane of enterocytes.
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Debnam ES, Levin ER (1975) Effects of fasting and semistarvation on the kinetics of active and passive sugar absorption across the small intestine in vivo. J Physiol 252:681–700
Debnam ES, Thomas CS (1984) The effect of fasting on the potential differences across the brush-border membrane of enterocytes in rat small intestine. J Physiol (Lond) 355:449–456
Esposito G, Faelli A, Capraro V (1967) Metabolism and transport phenomena in isolated intestine of normal and semistarved rats. Arch Int Physiol Biochim 75:601–608
Esposito G (1967) Intestinal absorption of sugar in semistarved rats. Proc Soc Exp Biol Med 125:452–455
Esposito G, Faelli A, Tosco M, Orsenigo MN, Battistessa R (1985) Age-related changes in rat intestinal transport ofd-glucose, sodium and water. Am J Physiol 294:G328-G334
Faelli A, Esposito G, Capraro V (1966) Intracellular concentration of sodium and glucose correlated with transport phenomena. Arch Sci Biol 50:234–241
Groseclose R, Hopfer U (1978) Small intestinal sugar and aminoacid transport in semistarvation. Membr Biochem 2:135–148
Hindmarsh JT, Kilby D, Ross B, Wiseman G (1967) Further studies on intestinal active transport during semistarvation. J Physiol (Lond) 188:207–218
Hopfer U, Sigrist-Nelson K, Amman E, Murer H (1976) Differences in neutral aminoacid and glucose transport between brush border and basal lateral plasma membrane of intestinal epithelial cells. J Cell Physiol 89:805–810
James PA, Mc Manus MB, Isselbacher KJ (1970) Effect of fasting versus feeding on the rat small intestine. Morphological, biochemical and functional differences. Gastroenterology 59:214–221
Kershaw TG, Neame KD, Wiseman G (1960) The effect of semistarvation on absorption by the rat small intestine in vitro and in vivo. J Physiol (Lond) 152:182–190
Kotler DP, Levine GM, Shiau Y-F (1980) Effects of nutrients, endogenous secretions, and fasting on in vitro glucose uptake. Am J Physiol 238:G219-G227
Lis MT, Crampton RF, Matthews DM (1972) Effect of dietary change on intestinal absorption ofl-methionine andl-methionyl-lmethionine in the rat. Br J Nutr 27:159–167
Madge DS (1970) Intestinal absorption of glucose and histidine isomers by fed and semistarved mice. Comp Biochem Physiol 32:1–6
Murer H, Hopfer U, Kinne-Saffran E, Kinne R (1974) Glucose transport in isolated brush border and basal-lateral plasma membrane vesicles from intestinal epithelial cells. Biochim Biophys Acta 345:170–179
Neale RJ, Wiseman G (1969) The use of dietary-restricted rat intestine for active transport studies. J Physiol (Lond) 205: 159–178
Newey H, Sanford PA, Smyth DH (1970) Effects of fasting on intestinal transfer of sugars and amino acids in vitro. J Physiol (Lond) 208:705–724
Orsenigo MN, Tosco M, Esposito G, Faelli A (1985) The basolateral membrane of rat enterocyte: its purification from brush border contamination. Anal Biochem 144:577–583
Rane F, Noriega R, Doffoel M, Grenier JF, Haffen K (1982) Modifications of brush border enzyme activities during starvation in the jejunum and ileum of adult rats. Enzyme 28:328–335
Steiner M, Gray SJ (1969) Effect of starvation on intestinal amino acid absorption. Am J Physiol 217:747–752
Sugiyama K, Yasumoto K, Mitsuda H (1977) Effect of semistarvation on kinetic parameters for the in vitro accumulation and transport ofl-leucine in rat small intestine. Agric Biol Chem 41:1969–1973
Wright CL, Barber HE (1969) The transport ofl-histidine across rat small intestine in vitro: an effect of semistarvation and energy substrate requirements. Biochem J 115:1075–1076
Wright EM, Van Os CH, Mircheff AK (1980) Sugar uptake by intestinal basolateral membrane vesicles. Biochim Biophys Acta 597:112–124
Yasumoto K, Sugiyama K, Mitsuda H (1977) Effects of semistarvation on the absorption ofd- andl-amino acids and on the electrical potential across rat small intestine in vitro. Agric Biol Chem 41:1961–1968
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Marciani, P., Lindi, C., Faelli, A. et al. Effects of semistarvation on transintestinald-glucose transport andd-glucose uptake in brush border and basolateral membranes of rat enterocytes. Pflugers Arch. 408, 220–223 (1987). https://doi.org/10.1007/BF02181462
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DOI: https://doi.org/10.1007/BF02181462