Pflügers Archiv

, Volume 408, Issue 3, pp 215–219 | Cite as

Procaine has opposite effects on passive Na and K permeabilities in frog skin

  • Maria-Luiza Flonta
  • Dagmar Galter
  • P. T. Frangopol
  • D. -G. Mărgineanu
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

Procaine has opposite effects on the active transport of Na+ when applied on the mucosal side of the frog skin [where it produces a stimulation of the short-circuit current (Isc)] or when added on the serosal side (where it produces an inhibition ofIsc). In an attempt to reveal and localize the primary effect of procaine on either the apical or latero-basal membranes of the epithelial cells, we have tried to “chemically dissect” both membrane functions with inhibitors and ionophores. When applied on the apical side of the latero-basally depolarized epithelium, 25 mmol/l procaine increasesIsc andVoc (transepithelial open-circuit potential), while decreasing the transepithelial resistance. TheE1E2 linearity domain of the I–V curves is narrowed. On the serosal side of the depolarized epithelium, the same concentration of procaine does not affectIsc andVoc (which are already inhibited) but it produces an increase in the transepithelial resistance (Rt). Procaine influence on the passive K+ permeability was studied by using the ionophore nystatin, which is assumed to form channels permeable to K+, when applied on the amiloride blocked apical membrane. In nystatin-treated epithelia, 25 mmol/l procaine on the apical side decreaseIsc,Voc andRt. In parallel experiments during Cl substitution by SO42−, the procaine effects onIsc andVoc are no longer maintained, but transient. The results suggest that procaine positively influences the Na+ transient through the apical Na+-channels, and inhibits the epithelial permeability for K+, possibly by reducing K+-ions accessibility to the K+-channels.

Key words

Passive-Na+ permeability Passive K+-permeability Procaine Frog skin Nystatin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ehrenfeld J, Garcia-Romeu F, Harvey BJ (1985) Electrogenic active proton pump inRana esculenta skin and its role in sodium ion transport. J Physiol 359:331–355Google Scholar
  2. 2.
    Flonta ML, Vais H, Frangopol PT, Mărgineanu DG (1985) Procaine effects on the sodium transport in frog skin. Arch Int Physiol Biochim 93:223–229Google Scholar
  3. 3.
    Frömter E, Sauer F (1985) Theoretical basis of electrophysiologic analysis of trnasepithelial transport. In: Seldin DW, Giebisch G (eds) The kidney: Physiology and pathophysiology. Raven Press, New York, pp 215–228Google Scholar
  4. 4.
    Fuchs W, Hviid Larsen E, Lindemann B (1977) Current-voltage curve of sodium channels and concentration dependence of sodium permeability in frog skin. J Physiol 267:137–166Google Scholar
  5. 5.
    Garty H (1984) Current-voltage relations of the baso-lateral membrane in tight amphibian epithelia: Use of nystatin to depolarize the apical membrane. J Membrane Biol 77:213–222Google Scholar
  6. 6.
    Helman SI, Fisher RS (1977) Microelectrode studies of the active Na transport pathway of frog skin. J Gen Physiol 69:571–604Google Scholar
  7. 7.
    Helman SI, Miller DA (1971) In vitro techniques for avoiding edge damage in studies of frog skin. Science 173:146–148Google Scholar
  8. 8.
    Hille B (1977) Hydrophilic and hydrophobic pathways for the drug-receptor reaction. J Gen Physiol 69:497–515Google Scholar
  9. 9.
    Kirschner LB (1983) Sodium chloride absorption across the body surface: frog skin and other epithelia. Am J Physiol 244:R429-R443Google Scholar
  10. 10.
    Koefoed-Johnsen V, Ussing HH (1958) The nature of the frog skin potential. Acta Physiol Scand 42:298–308Google Scholar
  11. 11.
    Kristensen P, Ussing HH (1985) Epithelial organization. In: Seldin DW, Giebisch G (eds) The kidney: Physiology and pathophysiology. Raven Press, New York, pp 173–188Google Scholar
  12. 12.
    Palmer LG, Edelman IS, Lindemann B (1980) Current-voltage analysis of apical sodium transport in toad urinary bladder: Effects of inhibitors of transport and metabolism. J Membrane Biol 57:59–71Google Scholar
  13. 13.
    Schwarz W (1980) Untersuchungen zur Blockierung der Nervenleitung durch Lokalanästhetika. Doctoral Thesis, Saarland UniversityGoogle Scholar
  14. 14.
    Skou JC, Zerahn K (1959) Investigations on the effect of some local anesthetics and other amines on the active transport of sodium through the isolated short-circuited frog skin. Biochim Biophys Acta 35:324–333Google Scholar
  15. 15.
    Tang J, Abramcheck FJ, Van Driessche W, Helman SI (1985) Electrophysiology and noise analysis of K+-depolarized epithelia of frog skin. Am J Physiol 249:C421-C429Google Scholar
  16. 16.
    Yeh J, Tanguy J (1985) Na channel activation gate modulates slow recovery from use-dependent block by local anesthetics in squid giant axons. Biophys J 47:685–694Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Maria-Luiza Flonta
    • 1
  • Dagmar Galter
    • 1
  • P. T. Frangopol
    • 2
  • D. -G. Mărgineanu
    • 1
  1. 1.Faculty of Biology, Biophysical LaboratoryUniversity of BucharestBucharest
  2. 2.Institute of Physics and Nuclear EngineeringBucharestRomania

Personalised recommendations