Journal of Applied Phycology

, Volume 2, Issue 4, pp 297–303 | Cite as

Callus culture ofPterocladia capillacea (Rhodophyta) and analysis of cell wall polysaccharides

  • Xue-Wu Liu
  • Cyrille Rochas
  • Bernard Kloareg
Article

Abstract

Calluses induced fromPterocladia capillacea have been kept in culture for more than three years. They exhibit a fast growth rate, owing to the release of single cells, which in turn develop into new callus. The effect of various media and culture conditions upon growth was investigated. In order to confirm the identity of the callus cells, a 0,45 mg incoculum was grown that yielded 15 g dried callus within six weeks. Polysaccharides from this material (5.5 g) were analysed by13C NMR spectroscopy. This produced a spectrum typical of agar and very similar to the one obtained for agar extracted fromP. capillacea plants. However, the callus agar displayed no gel-forming properties, even after alkali modification.

Key words

callus culture agar 13C NMR spectroscopy Pterocladia capillacea 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Xue-Wu Liu
    • 1
    • 2
  • Cyrille Rochas
    • 3
  • Bernard Kloareg
    • 2
  1. 1.School of Biological SciencesVictoria University of WellingtonWellingtonNew Zealand
  2. 2.Centre d'Etudes Océanologiques et de Biologie MarineCNRS UPR 4601 et Université Paris VIRoscoffFrance
  3. 3.Centre d'Etudes et de Recherches sur les Macromolécules Végétales, CNRSGrenoble cédexFrance

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