Abstract
A range of cell lines was isolated fromPorphyra umbilicalis L. (Rhodophyta) tissue using a variety of methods, the most successful involving exposure to a limpet acetone powder enzyme extract for 24 h, homogenisation and filtration through a series of polyester meshes. All established lines grew as 0.1–5 mm diameter aggregates in liquid culture; most were stable and have been grown in shake-flask or air-lift culture for periods in excess of 1 yr without reverting to the foliose growth form. An investigation of the medium used to grow these lines indicated that it was not nitrogen-deficient and that the sodium chloride concentration was optimal. The addition of an organic buffer increased the final cell yield. None of these cell lines grew heterotrophically in medium supplemented with a range of fixed carbon sources. The infrared spectra of polysaccharides isolated fromPorphyra aggregates and from tissue grown under identical conditions indicated that the structures of the two isolates were analogous.
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References
Arad SM, Friedman OD, Rotem A (1988) Effect of nitrogen on polysaccharide production in aPorphyridium sp. Appl. envir. Microbiol. 54: 2411–2414.
Bradley PM, Cheney DP, Saga N (1988) One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae. Pl. Cell Tissue Organ Cult. 12: 55–60.
Chen LCM (1986) Cell development ofPorphyra miniata (Rhodophyceae) under axenic culture. Bot. mar. 29: 435–439.
Chen LCM (1989) Cell suspension culture fromPorphyra linearis (Rhodophyta), a multicellular marine red alga. J. appl. Phycol. 1: 153–159.
Chen LCM, Taylor ARA (1978) Medullary tissue culture of the red algaChondrus crispus. Can. J. Bot. 56: 883–886.
Cheney DP, Luistro AH, Bradley PM (1987) Carrageenan analysis of tissue cultures and whole plants ofAgardhiella subulata. Hydrobiologia 151/152: 161–166.
Fries L (1963) On the cultivation of axenic red algae. Physiol. Pl. 16: 695–708.
Gibor A, Polne M, Biniaminov M, Neushul M (1981) Exploratory studies of vegetative propagation of marine algae: procedure for obtaining axenic tissues. Proc. Int. seaweed Symp. 10: 587–593.
Glicksman M (1987) Utilization of seaweed hydrocolloids in the food industry. Hydrobiologia 151/152: 31–47.
Imada O, Saito Y, Teramoto KI (1971) Artificial culture of laver. Proc. Int. seaweed Symp. 7: 358–363.
Liu XW, Gordon ME (1987) Tissue and cell culture of New ZealandPterocladia andPorphyra species. Hydrobiologia 151/152: 147–154.
Peat S, Turvey JR, Rees DA (1961) Carbohydrates of the red alga,Porphyra umbilicalis. J. chem. Soc. 1590–1595.
Polne M, Gibor A, Neushul M (1980) The use of ultrasound for removal of macroalgal epiphytes. Bot. mar 23: 731–734.
Polne-Fuller M, Gibor A (1984) Developmental studies inPorphyra. I. Blade differentiation inPorphyra perforata as expressed by morphology, enzymatic digestion and protoplast regeneration. J. Phycol. 20: 609–616.
Polne-Fuller M, Biniaminov M, Gibor A (1984) Vegetative propagation ofPorphyra perforata. Hydrobiologia 116/117: 308–313.
Rees DA, Conway E (1962) The structure and biosynthesis of porphyran: a comparison of some samples. Biochem. J. 84: 411–416.
Saga N, Uchida T, Sakai Y (1978) CloneLaminaria from single isolated cell. Bull jap. Soc. Sci. Fish. 44(1): 87.
Sandford PA, Baird J (1983) Industrial utilisation of polysaccharides. In Aspinall GO (ed.), The Polysaccharides, vol 2. Academic Press, N.Y. & London, 411–490.
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Presented at the XIIIth International Seaweed Symposium, University of British Columbia, Vancouver, Canada, August 1989.
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Tait, M.I., Milne, A.M., Grant, D. et al. Porphyra cell cultures: isolation, growth and polysaccharide production. J Appl Phycol 2, 63–70 (1990). https://doi.org/10.1007/BF02179770
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DOI: https://doi.org/10.1007/BF02179770