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Roux's archives of developmental biology

, Volume 204, Issue 5, pp 284–307 | Cite as

Distribution, classification, and development ofDrosophila glial cells in the late embryonic and early larval ventral nerve cord

  • Kei Ito
  • Joachim Urban
  • Gerhard Martin Technau
Original Article

Abstract

To facilitate the investigation of glial development inDrosophila, we present a detailed description of theDrosophila glial cells in the ventral nerve cord. A GAL4 enhancer-trap screen for glial-specific expression was performed. Using UAS-lacZ and UAS-kinesin-lacZ as reporter constructs, we describe the distribution and morphology of the identified glial cells in the fully differentiated ventral nerve cord of first-instar larvae just after hatching. The three-dimensional structure of the glial network was reconstructed using a computer. Using the strains with consistent GAL4 expression during late embryogenesis, we traced back the development of the identified cells to provide a glial map at embryonic stage 16. We identify typically 60 (54–64) glial cells per abdominal neuromere both in embryos and early larvae. They are divided into six subtypes under three categories: surface-associated glia (16–18 subperineurial glial cells and 6–8 channel glial cells), cortex-associated glia (6–8 cell body glial cells), and neuropile-associated glia (8–10 nerve root glial cells, 14–16 interface glial cells, and 3–4 midline glial cells). The proposed glial classification system is discussed in comparison with previous insect glial classifications.

Key words

Drosophila Central nervous system Glia GAL4 enhancer trap Classification 

Abbreviations

%NAP

Percent neuromere antero-posterior

%NML

Percent neuromere medio-lateral

%NVD

Percent neuromere ventro-dorsal

ac

Anterior commissure

act

Cell body fibre tracts to the anterior commissure

aCC

anterior corner cell AEL After egg laying

A-SPG

A subperineurial glial cell

B-SPG

B subperineurial glial cell

cbf

Cell body fibres

CBG

Cell body glia (glial cell)

CG

Channel glia (glial cell)

CNS

Central nervous system D Dorsal

DAB

3,3′-diaminobenzidine

DLSPG

Dorsal lateral subperineurial glial cell

dn

Dorsal nerve (transverse nerve)

DV

channel Dorsoventral channel

D-CG

Dorsal channel glial cell

D-IG

Dorsal interface glial cell (cluster)

D-MG

Dorsal midline glial cell (cluster)

DRG Dorsal

roofglia

EG

Exit glia (glial cell)

FETi

Fast extensor tibiae

GDA

glutardialdehyde

GFAP

Glial fibrillary acid protein

GIGI

Glial glia

IG

Interface glia (glial cell)

ISNG

Intersegmental nerve root glia (glial cell)

ISNR

Intersegmental nerve root

L

Lateral

L1

First instar larvae just after hatching

D-SPG

Lateral dorsal subperineurial glial cell

LG

longitudinal glia (glial cell)

LV-SPG

Lateral ventral subperineurial glial cell

L-CBG

Lateral cell body glial cell

L-IG

Lateral interface glial cell (cluster)

L-ISNG

Lateral intersegmental nerve root glial cell

L-SNG

Lateral segmental nerve root glial cell

M

medial

MD-SPG

Medial dorsal subperineurial glial cell

MG

midline glia (glial cell) (cluster)

MM

medialmost

MM-CBG

medialmost cell body glial cell

MNB

Median neuroblast

MV-SPG

Medial ventral subperineurial glial cell

m-cbf

Cell body fibre tracts from the midline neurons

M-CBG

Medial cell body glial cell

M-CG

Medial channel glial cell (cluster)

M-ISNG

Medial intersegmental nerve root glial cell

M-SNG

Medial segmental nerve root glial cell

NG

Nerve root glia (glial cell)

nho

Neurohemal organ

NPG

Neuropilar glia (glial cell)

PBS

phosphate-buffered saline

PBT

PBS with Triton-X

pc

Posterior commissure

pct

Cell body fibre tracts to the posterior commissure

PEM

PIPES-EGTA-Mg S04 buffer

PG

Peripheral glia (glial cell)

PNG

Perineurial glia (glial cell)

RT

room temperature

SaGl

Satellite glia

SBC

Segment boundary cell

SNG

Segmental nerve root glia (glial cell)

SNR

Segmental nerve root

SPG

Subperineurial glia (glial cell)

TpGl

Transport glia

TrGl

tracheal glia

V

Ventral

VL-CBG

Ventral lateral cell body glial cell

VI-SPG

Ventral lateral subperineurial glial cell

VNC

Ventral nerve cord

vt

Vertical cell body fibre tracts to the dorsal neuropile

VUM

Ventral unpaired median

V-CG

Ventral channel glial cell

V-IG

Ventral interface glial cell

V-MG

Ventral midline glial cell (cluster)

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Kei Ito
    • 1
  • Joachim Urban
    • 1
  • Gerhard Martin Technau
    • 1
  1. 1.Institut für Genetik, Universität MainzMainzGermany

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