Internationales Archiv für Arbeitsmedizin

, Volume 31, Issue 2, pp 123–134 | Cite as

Structural alterations and content of nicotinamide-adenine nucleotides in skeletal muscle of rat in chronic experimental carbon disulfide intoxication

  • Teresa Wrońska-Nofer
  • Jan Stetkiewicz
  • Stefan Szendzikowski


The histological nature and extent of muscular lesions and the content of nicotinamide-adenine nucleotides in the skeletal muscles of white rats exposed to CS2 at a concentration of 1.5 mg/l for 1 to 14 months were studied.

From the 5th month of exposure muscle atrophy of the denervation type was the constant histological finding. Five months later a significant fall in the nucleotide level was noted, parallel to apparent paresis of the hind limbs and gross muscular atrophy. No evidence of any inflammatory reaction or dystrophic myopathy was found. Reduction of the nucleotide content was attributed therefore to secondary alterations in the metabolism of skeletal muscle undergoing atrophy.

Key words

Carbon disulfide Rat muscle alterations 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Adams, R. D., Denny-Brown, D., Pearson, C. M.: Diseases of muscle. New York: Harper and Row 1962.Google Scholar
  2. 2.
    Alpers, B. J., Lewey, F. H.: Changes in the nervous system following carbon disulphide poisoning in animals and in man. Arch. Neurol. Psychiat. (Chic.)44, 725–739 (1940).Google Scholar
  3. 3.
    Bagiński, S.: Histological technique (Polish). Warszawa: PIW 1969.Google Scholar
  4. 4.
    Bloom, W., Fawcett, D. W.: Histologie (translated in Polish by K. Ostrowski). Warszawa: PZWL 1967.Google Scholar
  5. 5.
    Bosanquet, F. D., Daniel, P. M., Parry, H. B.: Myopathy: the pathological changes. The structure and function of muscle, Bourne, G. H., Ed., Vol. III, pp. 321–358. New York: Academic Press 1960.Google Scholar
  6. 6.
    Cohen, A. E., Scheel, L. D., Kopp, J. F., Stockell, F. R., Keenan, R. G., Montain, J. T., Paulus, H. J.: Biochemical mechanisms in chronic carbon disulphide poisoning. Amer. industr. Hyg. Ass. J.20, 303–323 (1959).Google Scholar
  7. 7.
    Drogiczina, E. A.: Toxic polyneuritis and encephalomyeloneuritis (Russian). Moskwa: Medgiz 1959.Google Scholar
  8. 8.
    Drury, R. A. B., Wallington, E. A.: Carleton's histological technique. New York: Oxford University Press 1967.Google Scholar
  9. 9.
    Hamilton, A.: Occupational poisoning in the visoces rayon industry. Washington: U.S. Gov. Print. Office 1940.Google Scholar
  10. 10.
    Hausmanowa-Petrusewicz, I.: Diseases of muscle (Polish). Warszawa: PZWL 1967.Google Scholar
  11. 11.
    Heydt, A.: Schwefelkohlenstoffpolyneuritis. Nervenarzt22, 93–99 (1951).Google Scholar
  12. 12.
    Kesy, I.: Colorimetric method of determining hydrogen sulphide and carbon disulphide in air (Polish). Med. Pracy1, 16–27 (1950).Google Scholar
  13. 13.
    Lewey, F. H.: Neurological, medical and biochemical signs and symptoms indicating chronic industrial disulphide absorption. Ann. intern. Med.15, 869–883 (1941).Google Scholar
  14. 14.
    Lewey, F. H.: Experimental chronic carbon disulphide poisoning in dogs. J. industr. Hyg.23, 415–436 (1941).Google Scholar
  15. 15.
    Liniecki, J.: The effect of chronic carbon disulphide poisoning on the thiamine balance in rats (Polish). Soc. Sc. łódźIV, No. 26 (1960).Google Scholar
  16. 16.
    Manu, P., Ruth, L., Lancranjan, I., Ionescu, S., Negau, F., Vasilescu, I.: Carbon disulphide toxic polyneuritis (Roumanien). Med. interna (Buc.)21, 303–312 (1969).Google Scholar
  17. 17.
    Manu, P., Ruth, I., Lancranjan, I., Ionescu, S., Negau, F., Vasilescu, I.: The value of electromyographic changes in the early diagnosis of carbon disulphide peripheral neuropathy. Med. d. Lavoro61, 102–108 (1970).Google Scholar
  18. 18.
    Sokal, J. A.: Intracellular distribution of pyridine nucleotides in the liver of rats after long-term exposure to carbon disulphide. Biochem. Pharmacol.17, 2489–2494 (1968).Google Scholar
  19. 19.
    Sokal, J. A., Tarkowski, S., Wrońska-Nofer, T.: A simplified method for determination of nicotinamide-adenine dinucleotides in liver tissue. Acta biochim. pol.16, 1–19 (1969).Google Scholar
  20. 20.
    Szendzikowski, S., Stetkiewicz, J., Wrońska-Nofer, T., Zdrajkowska, I.: Structural aspects of experimental carbon disulfide neuropathy. I. Development of neurohistological changes in chronically intoxicated rats. Int. Arch. Arbeitsmed.31, 135–149 (1973).Google Scholar
  21. 21.
    Szymczykiewicz, K.: Influence of vitamin B1 on the chronic carbon disulphide intoxication in albino rats (Polish). Med. Pracy7, 235–245 (1957).Google Scholar
  22. 22.
    Vigliani, E. C., Pernis, B.: L'intossicazione cronica da solfuro di carbonio. Atti Congr., Intern. Med. Lavoro (Napoli) 1954.Google Scholar
  23. 23.
    Wrońska-Nofer, T.: The influence of nicotinic acid upon the disturbances in lipid metabolism caused by carbon disulphide in rats. Int. Arch. Arbeitsmed.27, 221–227 (1970).Google Scholar
  24. 24.
    Wrońska-Nofer, T.: The relationship between the magnitude of the disturbances in lipid metabolism in rats and concentration of carbon disulphide in air. Med. d. Lavoro (in press).Google Scholar
  25. 25.
    Wrońska-Nofer, T., Sokal, J. A., Tarkowski, S.: The levels of nicotinamide-adenine nucleotides in the tissues of rat in the course of chronic carbon disulphide intoxication (Polish). Med. Pracy21, 249–254 (1970).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Teresa Wrońska-Nofer
    • 1
  • Jan Stetkiewicz
    • 1
  • Stefan Szendzikowski
    • 1
  1. 1.Department of Biochemistry and Department of PathomorphologyInstitute of Occupational MedicinełódźPoland

Personalised recommendations