Plant and Soil

, Volume 75, Issue 1, pp 75–85 | Cite as

Effects of high concentrations of sodium chloride and polyethylene glycol on the growth and ion absorption in plants

I. Water culture experiments in a greenhouse
  • T. Kawasaki
  • T. Akiba
  • M. Moritsugu


To provide a better understanding of plant responses to salt and water stresses, the effects of high concentrations of sodium chloride (NaCl) and polyethylene glycol (PEG) on the growth and ion absorption in plants were examined at similar osmotic potential conditions, using the water culture method. The inhibitory effect on plant growth appeared to be only slightly larger in PEG treatment than in NaCl treatment. However, the depressive effects on K, Ca and Mg contents of plants were more severe with NaCl treatment than with PEG treatment. No depressive effects of NaCl or PEG were observed for P content. From the short term absorption experiment using a tracer technique, it was also evident that the inhibition of K absorption was more drastic in NaCl treatment than in PEG treatment. On the other hand, the inhibitory effects of high concentrations of NaCl and PEG were very small on P absorption.

Key words

Bean Ion absorption Maize Phosphate-32 Plant growth Polyethylene glycol 86Rb Salt stress Sodium chloride Sorghum Water culture Water stress 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Awerbuch T and Avnimelech Y 1970 Counting of32P in plant tissues using the Cerenkov effect. Plant and Soil 33, 260–264.Google Scholar
  2. 2.
    Braunsberg H and Guyver A 1965 Automatic liquid scintillation counting of high-energy β emitters in tissue slices and aqueous solutions in the absence of organic scintillator. Anal. Biochem. 10, 86–95.Google Scholar
  3. 3.
    Bressan R A, Hasegawa P M and Handa A K 1981 Resistance of cultured higher plant cells to polyethylene glycol-induced water stress. Plant Sci. Lett. 21, 23–30.Google Scholar
  4. 4.
    Clausen T 1968 Measurement of32P activity in a liquid scintillation counter without the use of scintillator. Anal. Biochem. 22, 70–73.Google Scholar
  5. 5.
    Erlandsson G 1975 Rapid effects on ion and water uptake induced by changes of water potential in young wheat plants. Physiol. Plant. 35, 256–262.Google Scholar
  6. 6.
    Frota J N E and Tucker T C 1978 Salt and water stress influences nitrogen metabolism in red kidney beans. Soil Sci. Soc. Am. J. 42, 743–746.Google Scholar
  7. 7.
    Frota J N E and Tucker T C 1978 Absorption rates of ammonium and nitrate by red kidney beans under salt and water stress. Soil Sci. Soc. Am. J. 42, 753–756.Google Scholar
  8. 8.
    Fujinuma Y, Kinoshita A and Hashida S 1970 Salt concentration (in Japanese).In Analytical Methods of Soil Nutrients. Yokendo, Tokyo, pp 47–48.Google Scholar
  9. 9.
    Gupta U S 1977 Crop response to soil salinity and sodicity.In Physiological Aspects of Crop Nutrition and Resistance. Ed. U S Gupta. Atma Ram & Sons, Delhi, pp 316–317.Google Scholar
  10. 10.
    Haviland R T and Bieber L L 1970 Scintillation counting of32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic media. Anal. Biochem. 33, 323–334.Google Scholar
  11. 11.
    Jackson W T 1962 Use of carbowaxes (polyethylene glycols) as osmotic agents. Plant Physiol. 37, 513–519.Google Scholar
  12. 12.
    Jensen C R 1981 Influence of water and salt stress on water relationships and carbon dioxide exchange of top and roots in beans. New Phytol. 87, 285–295.Google Scholar
  13. 13.
    Läuchli A 1969 Radioassay for β-emitters in biological materials using Cerenkov radiation. Int. J. Appl. Rad. Isot. 20, 265–270.Google Scholar
  14. 14.
    Lawlor D W 1970 Absorption of polyethylene glycols by plants and their effects on plant growth. New Phytol. 69, 501–513.Google Scholar
  15. 15.
    Lupina L P 1966 Influence of high isoosmotic concentrations of dextran and sodium chloride on the nitrogen and carbon metabolism of corn. Sov. Plant Physiol. 13, 904–913.Google Scholar
  16. 16.
    Lupina L P 1967 Effect and after effect of high isoosmotic concentrations of NaCl and dextran on horse-bean plants. Sov. Plant Physiol. 14, 271–277.Google Scholar
  17. 17.
    Michel B E and Kaufmann M R 1973 The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51, 914–916.Google Scholar
  18. 18.
    Pannarun A M, Kovoor A and Heller R 1977 Cerenkov counting of24Na in suspension cultures—an economical use of scintillation apparatus for studying ion exchange. Physiol. Plant. 39, 323–327.Google Scholar
  19. 19.
    Sánchez-Díaz M, Aparicio-Tejo P, González-Murúa C and Peña J I 1982 The effect of NaCl salinity and water stress with polyethylene glycol on nitrogen fixation, stomatal response and transpiration ofMedicago sativa. Trifolium repens andTrifolium brachycalycinum (subclover). Physiol. Plant. 54, 361–366.Google Scholar
  20. 20.
    Slavik B 1974 Methods of Studying Plant Water Relations. Springer-Verlag, Berlin-Heidelberg-New York, pp 18–20.Google Scholar
  21. 21.
    Steuter A A, Mozafar A and Goodin J R 1981 Water potential of aqueous polyethylene glycol. Plant Physiol. 67, 64–67.Google Scholar
  22. 22.
    Storey R and Wyn Jones R G 1978 Salt stress and comparative physiology in the gramineae. I. Ion relations of two salt- and water-stressed barley cultivars. California Mariout and Arimar. Aust. J. Plant Physiol. 5, 801–816.Google Scholar
  23. 23.
    West D W, Merrigan I F, Taylor J A and Collins G M 1980 Growth of ornamental plants irrigated with nutrient or polyethylene glycol solutions of different osmotic potentials. Plant and Soil 56, 99–111.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1983

Authors and Affiliations

  • T. Kawasaki
    • 1
  • T. Akiba
    • 1
  • M. Moritsugu
    • 1
  1. 1.Institute for Agricultural and Biological SciencesOkayama UniversityKurashikiJapan

Personalised recommendations