Zusammenfassung
5-Hydroxytryptamin (Serotonin, Enteramin, Thrombocytin), das in neuerer Zeit aus Blut oder Gewebe verschiedener Tiere isoliert wurde, konnte nun auch unter Verwendung von Isotopentechnik und Papierchromatographie als unmethylierter Vorläufer der schon bekannten Krötenbasen im Giftextrakt vonBufo marinus im Ausmasse von 0,1 % (bezogen auf trockenen Giftextrakt) nachgewiesen werden.
References
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We wish to thank Dr.C. Bernard Lewis of the Institute of Jamaica for supplying the specimens ofBufo marinus.
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5-Hydroxytryptamine, as the creatinine sulfate complex, was generously supplied by the Abbott Laboratories, North Chicago, Illinois, U.S.A.
The radioactivity of the samples, which were essentially weightless, was measured with a 1.8 mg/cm2 mica window G. M. counter. The amounts of I131 and S35 in each sample were determined by measurement of radioactivity with and without an aluminum absorber as described byA. S. Keston, S. Udenfriend andM. Levy, J. Amer. Chem. Soc.72, 748 (1950) andS. Udenfriend, J. Biol. Chem.187, 65 (1950).
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Udenfriend, S., Clark, C.T. & Titus, E. The presence of 5-hydroxytryptamine in the venom ofBufo marinus. Experientia 8, 379–380 (1952). https://doi.org/10.1007/BF02176193
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DOI: https://doi.org/10.1007/BF02176193