Abstract
The entire chamber angle tissue of ten monkey eyes and nine normal human eyes was cultivated in organ cultures for 1–10 days and then investigated by electron microscopy. We found that the uveal and corneoscleral trabecular cells often degenerate as early as 2–3 days after explantation, whereas the cells of the cribriform region proliferate and show an increasing number of cell organelles (mitochondria, endoplasmic reticulum and Golgi complexes). Regarding the behaviour in vitro, we distinguished three different cell populations in the trabecular meshwork with probably different functions: (1) the endothelial cells of Schlemm's canal, (2) the trabecular cells of the cribriform region and (3) the uveal or corneoscleral trabecular cells.
Zusammenfassung
Kammerwinkelgewebe von 10 Affen- und 9 normalen menschlichen Augen wird als Organkultur 1–10 Tage in vitro gezüchtet und anschließend e.m. untersucht. Es zeigte sich, daß die uvealen und korneoskleralen Trabekelzellen häufig schon 2–3 Tage nach der Explantation zugrunde gehen, während die Zellen der kribriformen Region proliferieren und vermehrt Organellen (Mitochondrien, ER und Golgi-Komplexe) ausbilden. Entsprechend dem in vitro Verhalten werden 3 verschiedene Zellpopulationen im Trabekelwerk mit wahrscheinlich unterschiedlichen Funktionen unterschieden, und zwar 1. die Endothelzellen des Schlemm'schen Kanals, 2. die Trabekelzellen der kribriformen Region und 3. die uvealen bzw. korneoskleralen Trabekelzellen.
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References
Alvarado JA, Polansky JR (1979) Biologic activity of cultured human trabecular cells. Invest Ophthalmol Suppl (ARVO, Sarasota) p 241
Grierson I, Lee WR (1973) Erythrocyte phagocytosis in the human trabecular meshwork. Brit J Ophthalmol 57: 400–415
Grierson I, Robins E, Howes RC (1980) Preliminary observations on human trabecular meshwork cells in vitro. A v Graefes Arch Klin Exp Ophthalm 212: 173–186
Hassel JR, Newsome DA, Ballintine EJ (1980) Synthesis of extracellular matrix components by trabecular meshwork in organ culture. Invest Ophthalmol Suppl (ARVO, Orlando) p 273
Jung Y, Armaly MF (1979) In vitro studies on Rhesus monkey trabecular meshwork and acid mucopolysaccharides. Invest Ophthalmol Suppl (ARVO, Sarasota) p 241
Jung Y, Armaly MF (1980) Organ culture of trabeculectomy specimens of glaucomatous eyes. Invest Ophthalmol Suppl (ARVO, Orlando) p 273
Lütjen-Drecoll E (1972) Electron microscopic studies on reactive changes of the trabecular meshwork in human eyes after microsurgery. A v Graefes Arch Klin Exp Ophthalm 183: 267–285
Polansky JR, Gospodarowicz D, Weinreb R, Alvarado JA (1978) Human trabecular meshwork cell culture and glycosaminoglycan synthesis. Invest Ophthalmol Vis Res Suppl (ARVO) 17: 207
Polansky JR, Weinreb RN, Boxter JD, Alvarado JA (1979) Human trabecular cells. I. Establishment in tissue culture and growth characteristics. Invest Ophthalmol 18: 1043–1049
Polansky JW, Weinreb R, Alvarado JA (1981) Studies on human trabecular cells propagated in vitro. Vision Research 21: 155–160
Richardson TM (1979) Identification and distribution of glycosaminoglycans in the cat outflow system. Invest Ophthalmol Suppl (ARVO, Sarasota) p 240
Richardson TM (1980) Glycosaminoglycan synthesis in the cat trabecular meshwork. Invest Ophthalmol Suppl (ARVO, Orlando) p 274
Rohen JW, Unger HH (1959) Zur Morphologie und Pathologie der Kammerbucht des Auges. (Abh. der Math.-naturwiss. Klasse d. Akad. der Wissenschaften und der Lit., Mainz) Franz Steiner, Wiesbaden
Rohen JW, van der Zypen E (1968) The phagocytic activity of the trabecular meshwork endothelium. A v Graefes Arch Klin Exp Ophthalmol 175: 218–225
Rohen JW, Witmer R (1972) Electron microscopic studies on the trabecular meshwork in glaucoma simplex. A v Graefes Arch Klin Exp Ophthalm 183: 251–266
Rohen JW, Schachtschabel DO, Matthiessen P (1975) In vitro studies on the trabecular meshwork of the primate eye. A v Graefes Arch Klin Exp Ophthalm 193: 95–107
Rohen JW, Schachtschabel DO, Figge H, Bigalke B (1976) Die Struktur der Kammerwasserabflußwege und ihre Veränderungen beim Glaukom: In vivo- und in vitro-Untersuchungen. Glaukom-Symposium Würzburg 1974, p 1–12, Enke, Stuttgart
Rohen JW, Schachtschabel DO (1978) Morphological and biochemical studies of the human trabecular meshwork in tissue culture. Invest Ophthalmol Suppl (ARVO, Sarasota) p 207
Rohen JW, Lütjen-Drecoll E (1981) Ageing and Non-ageing processes within the connective tissues of the anterior segment of the eye. In: Biochemical and Morphological Aspects of Ageing (Eds. WEG Müller and JW Rohen), Franz Steiner, Wiesbaden, p 157–172
Schachtschabel DO, Bigalke B, Rohen JW (1977) Production of glycosaminoglycans by cell cultures of the trabecular meshwork of the primate eye. Exp Eye Res 24: 71–80
Schachtschabel DO, Wever J, Rohen JW, Bigalke B (1981) Changes in glycosaminoglycan synthesis during in vitro ageing of cultured WI-38-cells and trabecular meshwork cells of the primate eye. In Biochemical and Morphological Aspects of Ageing (Eds WEG Müller and JW Rohen), Franz Steiner, Wiesbaden, p 175–185
Schachtschabel DO, Rohen JW, Wever J, Wehrmann R (1982) The trabecular meshwork in tissue culture. In: Basic Aspects of Glaucoma Research. Internat Sympos, Erlangen 1981 (Edit E Lütjen-Drecoll), Schattauer, Stuttgart, (in press)
Schachtschabel DO, Rohen JW, Wever J, Sames K (1982) Synthesis and composition of glycosaminoglycans by cultured human trabecular meshwork cells. Albr v Graefes Arch Klin Exp Ophthalmol 218: 113–117
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Supported by the Deutsche Forschungsgemeinschaft, 5300 Bonn-Bad Godesberg (Ro 81/17)
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Rohen, J.W., Schachtschabel, D.O. & Wehrmann, R. Structural changes of human and monkey trabecular meshwork following in vitro cultivation. Graefe's Arch Clin Exp Ophthalmol 218, 225–232 (1982). https://doi.org/10.1007/BF02175887
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DOI: https://doi.org/10.1007/BF02175887