Abstract
In the rat, continous exposure to so-called “cold light” of 800 lux leads to a nearly complete loss of retinal photoreceptors, which occurs within 4 weeks. This process is followed by vascularization of the retinal pigment epithelium (RPE). The origin, extent, and expansion of such proliferations within 6 months after the onset of irradiation were evaluated by means of light microscopy, transmission electron microscopy, scanning electron microscopy, corrosion cast preparation, india ink injection, and trypsin digest preparation. The vessels within the RPE were found to be fenestrated and to form a network with the vessels of the neurosensory retina. Invasion of Bruch's membrane and formation of chorioretinal anastomoses were not observed. The occurrence of vessels within the RPE coincided with a massive regression of capillaries in the neurosensory retina.
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References
Augsburger JJ, Benson WE (1980) Subretinal neovascularization, in chronic uveitis. Graefe's Arch Clin Exp Ophthalmol 215:43–51
Bellhorn RW, Burns MS, Benjamin JV (1980) Retinal vessel abnormalities of phototoxis retinopathy in rats. Invest Ophthalmol Vis Sci 19:584–595
Dalton AG (1955) A chrome-osmium fixation for electron microscopy. Anat Rec 121:281
De Mott DW, Davis TP (1959) Irradiance threshold for chorioretinal lesions. Arch Ophthalmol 62:653–656
Dollery CT, Bulpitt CJ, Kohner EM (1969) Oxygen supply to the retina from the retinal and choroidal circulations at normal and increased arterial oxygen tensions. Invest Ophthalmol 8: 588–594
El-Hifnawi E (1984) Pathomorphologische Untersuchungen zum Verlauf der hereditären Netzhaut-Dystrophie bei R.C.S.-Ratten. Enke, Stuttgart
El-Hifnawi E, Koch F (1982) Licht- und elektronenmikroskopische Untersuchungen an retinalen und chorioidalen Gefäßen bei Wistar-Ratten. Verh Anat Ges 76:425–426
Francois J, De Laey JJ, Cambie E, Hanssens M, Victoria-Troncoso V (1975) Neovascularization after Argon laser photocoagulation of macular lesions. Am J Ophthalmol 79:206–210
Friedman E, Smith TR, Kuwabara T (1963) Senile choroidal vascular patterns and drusen. Arch Ophthalmol 69:220–230
Geeraets WJ, Ridgeway D (1963) Retinal damage from high intensity light. Acta Ophthalmol 76:109–112
Grignolo A, Orzalesi N, Castellazzo R, Vittone P (1969) Retinal damage by visible light in albino rats: an electron microscope study. Ophthalmologica 157:43–59
Grindle CFJ, Marshall J (1978) Ageing changes in Bruch's membrane and their functional implications. Trans Ophthalmol Soc UK 98:172–175
Ham WT, Ruffolo JJ, Mueller HA, Clarke AM, Moon ME (1978) Histologic analysis of phototechnical lesions produced in Rhesus retina by short-wave-length light. Invest Ophthalmol Vis Sci 17:1029–1035
Ham WT, Mueller HA, Ruffolo JJ, Clarke AM (1979) Sensitivity of the retina to radiation damage as a function of wavelength. Photochem Photobiol 29:735–743
Hodde KC, Miodonski A, Bakker C, Veltman WAM (1977) Scanning electron microscopy of microcorrosion casts with special attention on arterio-venous differences and application to the rat's cochlea. Scan Electron Microsc 2:477–484
Howell WL, Rapp LM, Williams TP (1982) Distribution of melanosomes across the retinal pigment epithelium of a hooded rat: implications for light damage. Invest Ophthalmol Vis Sci 22: 139–144
Kuwabara T, Cogan DG (1960) Studies of retinal vascular patterns. Arch Opthalmol 64:124/904–131/911
Kuwabara T, Gorn RA (1968) Retinal damage by visible light. An electron microscopic study. Arch Ophthalmol 79:69–78
Lai Y-L, Jacoby RO, Jonas AM (1978) Age-related and light-associated retinal changes in Fischer-rats. Invest Ophthalmol Vis Sci 17:634–638
Lanum J (1978) The damaging effects of light on the retina. Empirical findings, theoretical and practical implications. Surv Ophthalmol 22:221–249
Lawwill R, Crockett S, Currier G (1977) Retinal damage secondary to chronic light exposure, thresholds and mechanisms. Doc Ophthalmol 44:379–402
Lincoff H, Kreissig I (1979) Cryogenic and thermal effects on the retinal pigment epithelium. In: Zinn KM, Marmor MF (eds) The retinal pigment epithelium. Harvard University Press, Cambridge, Mass, London, pp 314–333
Marshall J, Hamilton AM, Bird AC (1975) Histopathology of Ruby and Argon laser lesion in monkey and human retina. Br J Ophthalmol 59:610–630
Miodonski A, Hodde KC, Bakker C (1976) Rasterelektronenmikroskopie von Plastik-Korrosions-Präparaten: Morphologische Unterschiede zwischen Arterien und Venen. Beitr Elektronenmikrosk Direktabb Oberfl 9:435–442
Noell WK, Walker VS, Kang BS, Berman S (1966) Retinal damage by light in rats. Invest Ophthalmol 5:450–473
Noell WK (1980) There are different kinds of retinal light damage in the rat. In: Williams TP, Baker BN (eds) Effects of constant light. Plenum Press, New York London, pp 3–28
O'Steen WK, Shear CR, Anderson KV (1972) Retinal damage after prolonged exposure to visible light: a light and electron microscopic study. Am J Anat 134:5–22
Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:208–212
Sachs L (1974) Angewandte Statistik, 4th edn. Springer, Berlin Heidelberg New York
Sarks SH (1973) New vessel formation beneath the retinal pigment epithelium in senile eyes. Br J Ophthalmol 57:951–965
Spitznas M, Bornfeld N (1978) The architecture of the most peripheral retinal vessels. Graefe's Arch Clin Exp Ophthalmol 208:125–133
Tanaka K, Naguro T (1981) High resolution scanning electron microscopy of cell organelles by a new specimen preparation method. Biomed Res 2:63–70
Teeters VW, Brid AC (1973) A clinical study of the vascularity of senile disciform macular degeneration. Am J Ophthalmol 75:53–65
Ts'o MOM, Wallow IHL, Powell JO (1973) Differential susceptibility of rod and cone cells to Argon laser. Arch Ophthalmol 89:228–234
Verhoeff FH, Grossman HP (1937) Pathogenesis of disciform degeneration of the macula. Arch Ophthalmol 18:561–585
Wallow IHI, Engerman RL (1977) Permeability and patency of retinal blood vessels in experimental diabetes. Invest Ophthalmol Vis Sci 16:447–461
Wallow IHI, Geldner PS (1980) Endothelial fenestrae in proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci 19:1176–1183
Weiter JJ, Zuckerman R (1980) The influence of the photoreceptor-RPE complex on the inner retina. An explanation for the beneficial effects of photo-coagulation. Ophthalmology 11:1133–1139
Weizenbaum F, Colavita F (1975) Behavioral and electrophysiological changes in visual sensitivity following prolonged exposure to constant light. Exp Neurol 48:440–446
Williams TP, Baker BN (1980) The effect of constant light. Plenum Press, New York London
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Koch, F., El-Hifnawi, E.S. & Spitznas, M. The effect of long-term irradiation with fluorescent light on the rat fundus. Graefe's Arch Clin Exp Ophthalmol 225, 226–234 (1987). https://doi.org/10.1007/BF02175454
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DOI: https://doi.org/10.1007/BF02175454