Abstract
In vivo cannulation of retinal blood vessels has not been reported but would be useful in exploring local vascular parameters and new treatment modalities. We developed a microsurgical technique for cannulating rabbit and cat retinal vessels, using glass micropipettes with curved shanks made from standard capillary tubing. The micropipette was held by a micromanipulator, which was fitted with a fiberoptic sleeve for intraocular illumination and for micropipette tip protection during insertion through the sclera. Direct aspiration from and injection into retinal blood vessels were possible.
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Supported by Duke University Core Grant (1 P30 EYO5722), Medical Research Funds from the Veterans Administration, a Fight for Sight Student Fellowship, c/o Mr. Bob Hope, Fight for Sight, Inc., New York, and the National Society to Prevent Blindness, New York
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Allf, B.E., de Juan, E. In vivo cannulation of retinal vessels. Graefe's Arch Clin Exp Ophthalmol 225, 221–225 (1987). https://doi.org/10.1007/BF02175453
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DOI: https://doi.org/10.1007/BF02175453