Abstract
β-Carotene, canthaxanthin, and β-carotene plus canthaxanthin were administered to “chinchilla bastard” pigmented rabbits in their rabbit diet (approximately 200 ppm carotenoid per group). The effect of the carotenoids on retinal function and morphology was tested against a control group in the course of 11 months. Electroretinography showed that in contrast to the control animals, β-carotene-treated rabbits produced increasing peak latencies of the scotopicb-waves. In the canthaxanthin-treated rabbits,a- andb-waves showed hypernormal amplitudes at low cumulative dosages (approximately 0.5–2 g) and reduced amplitudes at higher dosages (about 5 g). The peak latencies of the scotopica- andb-waves increased remarkably. This effect was still stronger in the carotenoid combination. Histology and electron microscopy indicated that in contrast to the control animals, canthaxanthin-treated rabbits showed a reduction in retinal thickness in some samples. In particular, they exhibited alterations in the granular layers and a marked diminuation of the photoreceptor outer segments and morphological alterations of the photoreceptor inner segments with massive deposition of electron-dense material. In all animals treated with carotenoids, lipid droplets of the retinal pigment epithelium were enlarged in size and number.
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Bok D, Ong DE, Chytil P (1984) Immunocytochemical localization of cellular retinol binding protein in the rat retina. Invest Ophthalmol Vis Sci 25:877–883
Boudreault G, Cortin P, Corriveau LA, Rousseau AP, Tardif Y, Malenfant M (1983) La rétinopathie à la canthaxanthine. I. Étude clinique de 51 consommateurs. Can J Ophthalmol 18:325–328
Bridges CDB, O'Gorman S, Fong SL, Alvarez RA, Berson E (1985) Vitamin A and interstitial retinol-binding protein in an eye with excessive retinitis pigmentosa. Invest Ophthalmol Vis Sci 26:684–691
Carter-Dawson L, Kuwabara T, O'Brien PJ, Bieri J (1979) Structural and biochemical changes in vitamin A-deficient rat retinas. Invest Ophthalmol Vis Sci 18:437–446
Carter-Dawson L, Kuwabara T, Bieri JG (1982) Intrinsic, light-dependent, regional differences in photoreceptor cell degeneration in vitamin A-deficient rat retinas. Invest Ophthalmol Vis Sci 22:249–252
Cortin P, Corriveau LA, Rousseau AP, Tardif Y, Malenfant M, (1982) Maculopathie en pailettes d'or. Can J Ophthalmol 17:103–106
Cortin P, Boudreault G, Rousseau AP, Tardif Y, Malenfant M (1984) La rétinopathie à la canthaxanthin. II. Facteurs prédisposants. Can J Ophthalmol 19:215–219
De Vries-De Mol EC, Went LN, Volker-Dieben HJ (1974) Farnsworth-Munsell 100-hue results in a series of patients with long-standing therapeutic carotenemia. Mod Probl Ophthalmol 13: 349–352
Gahl WA, Tietze F, Bashan N, Steinherz R, Schulman JD (1982) Defective cystine exodus from isolated lysosome-rich fractions of cystinotic leucocytes. J Biol Chem 257:9570–9575
Hollyfield JG, Fliesler SJ, Rayborn ME, Fong SL, Landers RA, Bridges CD (1985) Synthesis and secretion of interstitial retinol-binding protein by the human retina. Invest Ophthalmol Vis Sci 26:58–67
Jonas AJ, Smith ML, Schneider JA (1982) ATP-dependent lysosomal efflux is defective in cystinosis. J Biol Chem 257:13,185–13,188
Krastel H, Kretz J, Jaeger W (1985) Tritostörung bei Canthaxanthin-Einnahme. Wissenschaftliche Ausstellung der DOG, Heidelberg
Metge P, Mandriac-Bonnefroy C, Bellaube P (1984) Thésaurismose rétinienne à la canthaxanthine. Bull Mem Soc Fr Ophthalmol 95:547–549
Robison WG, Kuwabara T (1977) Vitamin A storage and peroxisomes in retinal pigment epithelium and liver. Invest Ophthalmol Vis Sci 16:1110–1117
Ros AM, Leyon H, Wennersten G (1985) Crystalline retinopathy in patients taking an oral drug containing canthaxanthine. Photodermatology 2:183–185
Schmitz U, Kluxen G, Weber U, Wegener A, Rink H (1987) Dimethylsulfoxide induced side effects on the rabbit eye. Symposium on drug-induced side effects and ocular toxicity, Bonn (in press)
Weber U, Goerz G, Hennekes R (1985a) Carotinoid-Retinopathie. I. Morphologische und funktionelle Befunde bei 30 carotinoidbehandelten Patienten. Klin Monatsbl Augenheilkd 186: 351–354
Weber U, Hennekes R, Goerz G (1985b) Carotinoid-Retinopathie. II. Elektrophysiologische Befunde bei 23 carotinoid-behandelten Patienten. Klin Monatsbl Augenheilkd 187:507–511
Weber U, Goerz G (1986) Carotinoid-Retinopathie. III. Reversibilität. Klin Monatsbl Augenheilkd 188:20–22
Weber U, Sons U, Hennekes R, Lenz W (1986) Experimental calcium oxalate retinopathy compared with cystine- and tyrosine-induced retinal damage. Graefe's Arch Clin Exp Ophthalmol 224:414–418
Weleber RG, Denman ST, Hanifin JM, Cunningham WJ (1986) Abnormal retinal function associated with isotretinoin therapy for acne. Arch Ophthalmol 104:831–837
Wong VG, Kuwabara T, Brubaker R, Olson W, Schulman J, Seegmiller JE (1970) Lysosomal cystine crystals in cystinosis. Invest Ophthalmol Vis Sci 9: 83–88
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Weber, U., Kern, W., Novotny, G.E.K. et al. Experimental carotenoid retinopathy. Graefe's Arch Clin Exp Ophthalmol 225, 198–205 (1987). https://doi.org/10.1007/BF02175449
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DOI: https://doi.org/10.1007/BF02175449