Abstract
Phosphoenolpyruvate (PEP)-dependent phosphorylation experiments have indicated that the grampositive bacteriumStaphylococcus carnosus possesses an EIICBA fusion protein specific for glucose. Here we report the cloning of a 7 kb genomic DNA fragment containing two genes,glcA andglcB, coding for the glucose-specific PTS transporters EIIGlc1 and EIIGlc2 which are 69% identical. The translation products derived from the nucleotide sequence consist of 675 and 692 amino acid residues and have calculated molecular weights of 73 025 and 75 256, respectively. Both genes can be stably maintained inEscherichia coli cells and restore the ability to ferment glucose toptsG deletion mutants ofE. coli. This demonstrates the ability of the PTS proteins HPr and/or EIIAGlc of a gram-negative organism (E. coli) to phosphorylate an EIICBAGlc from a gram-positive organism (S. carnosus).
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Communicated by J. W. Lengeler
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Christiansen, I., Hengstenberg, W. Cloning and sequencing of two genes fromStaphylococcus carnosus coding for glucose-specific PTS and their expression inEscherichia coliK-12. Molec. Gen. Genet. 250, 375–379 (1996). https://doi.org/10.1007/BF02174396
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DOI: https://doi.org/10.1007/BF02174396