Abstract
A popular method for the investigation of transcriptional regulation of gene expression is direct measurement of mRNA levels. As an internal control the level of a transcript from a constitutively expressed gene is often determined. To measure the induction rate of stress-responsive genes fromBacillus subtilis the transcript of the constitutively expressedxynA gene was used as a control. But the results presented in this communication prove that the degradation rate of thexynA transcript rises considerably in response to different kinds of stress. This response to stress is not dependent on protein biosynthesis.
References
Haldenwang WG (1995) The sigma factors ofBacillus subtilis. Microbiol Rev 59:1–30
Hames BD, Higgins SJ (1985) Nucleic acid hybridisation: a practical approach. IRL Press, Oxford, pp 139–160
Henkin T, Sonenshein A (1987) Mutations of theEscherichia coli lacUV5 promoter resulting in increased expression inBacillus subtilis. Mol Gen Genet 209:467–474
Krüger E, Völker U, Hecker M (1994) Stress induction ofclpC inBacillus subtilis and its involvement in stress tolerance. J Bacteriol 176:3360–3367
Lindner C, Stülke J, Hecker M (1994) Regulation of xylanolytic enzymes inBacillus subtilis. Microbiology 140:753–757
Melin L, Rutberg L, von Gabain A (1989) Transcriptional and posttranscriptional regulation of theBacillus subtilis succinate dehydrogenase operon. J Bacteriol 171:2110–2115
Msadek T, Kunst F, Rapoport G (1994) MecB ofBacillus subtilis a member of the ClpC ATPase family is a pleiotropic regulator controlling competence gene expression and growth at high temperature. Proc Natl Acad Sci USA 91:5788–5792
Neidhardt FC, Bloch PL, Smith DF (1974) Culture medium for enterobacteria. J Bacteriol 119:736–747
Nicholson WL, Park YK, Henkin TM, Won M, Weikert MJ, Gaskel JA, Chambliss GH (1987) Catabolite responsive-resistant mutations of theBacillus subtilis alpha-amylase promoter affect transcription levels and are in an operator-like sequence. J Mol Biol 198:609–618
Nyström T, Neidhardt FC (1992) Cloning mapping and nucleotide sequencing of a gene encoding a universal stress protein inEscherichia coli. Mol Microbiol 6:3187–3198
Padmanabha KP, Deutscher MP (1991) RNase T affectsEscherichia coli growth and recovery from metabolic stress. J Bacteriol 173:1376–1381
Paice MG, Bourbonnais R, Desrochers M, Jurasek L, Yaguchi M (1986) A xylanase gene fromBacillus subtilis: nucleotide sequence and comparison withB. pumilus gene. Arch Microbiol 144:201–206
Resnekov O, Rutberg L, von Gabain A (1990) Changes in the stability of specific mRNA species in response to growth stage inBacillus subtilis. Proc Natl Acad Sci USA 87:8355–8359
Riethdorf S, Völker U, Gerth U, Winkler A, Engelmann S, Hecker M (1994) Cloning nucleotide sequence and expression of theBacillus subtilis lon gene. J Bacteriol 176:6518–6527
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Habor, New York
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Communicated by W. Goebel
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Allmansberger, R. Degradation of theBacillus subtilis xynA transcript is accelerated in response to stress. Molec. Gen. Genet. 251, 108–112 (1996). https://doi.org/10.1007/BF02174351
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DOI: https://doi.org/10.1007/BF02174351