Molecular and General Genetics MGG

, Volume 251, Issue 1, pp 99–107 | Cite as

Sequence and characterisation of a ribosomal RNA operon fromAgrobacterium vitis

  • L. Otten
  • P. De Ruffray
  • P. de Lajudie
  • B. Michot
Original Paper


One of the four ribosomal RNA operons (rrnA) from theAgrobacterium vitis vitopine strain S4 was sequenced.rrnA is most closely related to therrn operons ofBradyrhizobium japonicum andRhodobacter sphaeroides and carries an fMet-tRNA gene downstream of its 5S gene, as in the case ofR. sphaeroides. The 16S rRNA sequence of S4 differs from theA. vitis K309 type strain sequence by only one nucleotide, in spite of the fact that S4 and K309 have very different Ti plasmids. The predicted secondary structure of the S4 23S rRNA shows several features that are specific for the alpha proteobacteria, and an unusual branched structure in the universal B8 stem. The 3′ ends of the three otherrrn copies of S4 were also cloned and sequenced. Sequence comparison delimits the 3′ ends of the four repeats and defines two groups:rrnA/rrnB andrrnC/rrnD.

Key words

Ribosomal genes Agrobacterium Evolution 23S rRNA 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • L. Otten
    • 1
  • P. De Ruffray
    • 1
  • P. de Lajudie
    • 2
  • B. Michot
    • 3
  1. 1.Institute of Plant Molecular Biology of the CNRSStrasbourg CedexFrance
  2. 2.Laboratory of Microbiology ORSTOMDakarSenegal
  3. 3.Laboratory of Molecular Biology of Eucaryotes of the CNRSToulouse CedexFrance

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