Abstract
The impact of the codon bias of the mouse tumour necrosis factor α(mTNF) gene cloned inStreptomyces lividans on the efficiency of expression and secretion was analysed. Minor codons occurring in the mTNF gene were therefore adapted to the codon bias ofStreptomyces by site-directed mutagenesis. No improvement in mTNF yield could be detected. The stability of the transcript derived from the construct was shown to be more important for determining the final level of mTNF production. A strong correlation was observed between the yield of secreted biologically active mTNF and the amount of mTNF mRNA present in the cells.
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Communicated by C. A. M. J. J. van den Hondel
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Lammertyn, E., Van Mellaert, L., Bijnens, A.P. et al. Codon adjustment to maximise heterologous gene expression inStreptomyces lividans can lead to decreased mRNA stability and protein yield. Molec. Gen. Genet. 250, 223–229 (1996). https://doi.org/10.1007/BF02174182
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DOI: https://doi.org/10.1007/BF02174182