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Molecular and General Genetics MGG

, Volume 250, Issue 2, pp 214–222 | Cite as

chs-4, a class IV chitin synthase gene fromNeurospora crassa

  • Adi Beth Din
  • Charles A. Specht
  • Phillips W. Robbins
  • Oded Yarden
Original Paper

Abstract

InSaccharomyces cerevisiae, most of the cellular chitin is produced by chitin synthase III, which requires the product encoded by theCSD2/CAL1/DIT101/KT12 gene. We have identified, isolated and structurally characterized aCSD2/CAL1/DIT101/KT12 homologue in the filamentous ascomyceteNeurospora crassa and have used a “reverse genetics” approach to determine its role in vivo. The yeast gene was used as a heterologous probe for the isolation of aN. crassa gene (designatedchs-4) encoding a polypeptide belonging to a class of chitin synthases which we have designated class IV. The predicted polypeptide encoded by this gene is highly similar to those ofS. cerevisiae andCandida albicans. N. crassa strains in whichchs-4 had been inactivated by the Repeat-Induced Point mutation (RIP) process grew and developed in a normal manner under standard growth conditions. However, when grown in the presence of sorbose (a carbon source which induces morphological changes accompanied by elevated chitin content), chitin levels in thechs-4 RIP strain were significantly lower than those observed in the wild type. We suggest that CHS4 may serve as an auxiliary enzyme inN. crassa and that, in contrast to yeasts, it is possible that filamentous fungi may have more than one class IV chitin synthase.

Key words

Neurospora Ascomycete Fungal cell wall 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Adi Beth Din
    • 1
  • Charles A. Specht
    • 2
  • Phillips W. Robbins
    • 2
  • Oded Yarden
    • 1
  1. 1.Department of Plant Pathology and Microbiology, Faculty of AgricultureThe Hebrew University of JerusalemRehovotIsrael
  2. 2.Center for Cancer Research, Massachusetts Institute of TechnologyCambridgeUSA

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