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Molecular analysis of thescrA andscrB genes fromKlebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme IIScr of the phosphotransferase system and a sucrose-6-phosphate invertase

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Abstract

TheKlebsiella pneumoniae genesscrA andscrB are indispensable for sucrose (Scr) utilisation. GenescrA codes for an Enzyme IIScr (IIScr) transport protein of the phosphoenolpyruvate-dependent carbohydrate: phosphotransferase system (PTS), whilescrB encodes a sucrose 6-phosphate specific invertase. A 3.7 kbscrAB DNA fragment has been cloned fromK. pneumoniae and expressed inEscherichia coli. Its nucleotide sequence was determined and the coding regions forscrA (1371 bp) andscrB (1401 bp) were identified by genetic complementation, enzyme activity tests and radiolabelling of the gene products. In addition, the nucleotide sequence of thescrB gene from the conjugative plasmid pUR400 isolated fromSalmonella typhimurium was also determined and errors in the previously published sequence of thescrA gene of pUR400 were corrected. Extensive similarity was found between the sequences of ScrA and other Enzymes II, as well as between the two invertases and other sucrose hydrolysing enzymes. Based on the analysis of seven IIScr proteins, a hypothetical model of the secondary structure of IIScr is proposed.

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Authors and Affiliations

  1. Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands

    F. Titgemeyer

  2. Fachbereich Biologie/Chemie, Universität Osnabrück, Arbeitsgruppe Genetik, D-49069, Osnabrück, FRG

    K. Jahreis & J. W. Lengeler

  3. Department of Protein Therapeutics, Human Genome Sciences Inc., 9620 Medical Center Drive, # 300, 20850-3338, Rockville, Maryland, USA

    R. Ebner

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  1. F. Titgemeyer
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  2. K. Jahreis
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  3. R. Ebner
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  4. J. W. Lengeler
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Communicated by H. Böhme

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Titgemeyer, F., Jahreis, K., Ebner, R. et al. Molecular analysis of thescrA andscrB genes fromKlebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme IIScr of the phosphotransferase system and a sucrose-6-phosphate invertase. Molec. Gen. Genet. 250, 197–206 (1996). https://doi.org/10.1007/BF02174179

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  • DOI: https://doi.org/10.1007/BF02174179

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