Abstract
The structural gene (trxA) coding for thioredoxin in the photosynthetic bacteriumRhodobacter sphaeroides has been cloned and sequenced previously. In the present study, the role of oxygen intrxA expression inR. sphaeroides Y was investigated using mRNA analyses and plasmid-bornetrxA'-lacZ + translational and transcriptional fusions. Northern analysis revealed atrxA-specific transcript of approximately 420–460 nucleotides, indicating thattrxA is transcribed as a single gene. By studying the β-galactosidase activity in strains harboring various φ(trxA'-lacZ +) fusion constructs, the promoter region of thetrxA gene was localized within a 64-bp region located 97 nucleotides upstream of thetrxA initiator codon. A singletrxA transcription initiation site was mapped by primer extension, 27 bp upstream of thetrxA gene. Based on these results and DNA sequence analysis, we propose that a σ70 consensus sequence serves as atrxA promoter. Results from oxygen shift experiments, as deduced from both mRNA analysis and fusions of thetrxA promoter region tolacZ indicate that transcription of theR. sphaeroides trxA gene is regulated by high oxygen tension. DNA sequences involved in this oxygen regulation were also localized in the 64-bp region containing thetrxA promoter. Based on our finding, the hypothetical biological function of thioredoxin fromR. sphaeroides is discussed.
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Communicated by J. W. Lengeler
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Pasternak, C., Assemat, K., Breton, A.M. et al. Expression of the thioredoxin gene (trxA) inRhodobacter sphaeroides Y is regulated by oxygen. Molec. Gen. Genet. 250, 189–196 (1996). https://doi.org/10.1007/BF02174178
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DOI: https://doi.org/10.1007/BF02174178