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Organization of the histone H3 genes in soybean, barley and wheat

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Abstract

Several variants of the replacement histone H3 genes from soybean, barley and wheat have been cloned and sequenced. Analysis of segregating populations in barley and soybean, as well as analysis of clones isolated from a soybean genomic library, suggested that these genes are dispersed throughout the genome. Several genes contain introns located in similar positions, but of different lengths and sequence. Comparison of mRNA levels in different tissues revealed that the intron-containing and intronless genes have different expression patterns. The distribution of the introns in the histone H3 genes across several plant species suggests that some of the introns might have been lost during the evolution of the gene family. Sequence divergence among introns and gene-flanking sequences in cloned gene variants allowed us to use them as specific probes for localizing individual gene copies and analyzing the genomic distribution of these variants across a range of genotypes.

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Authors and Affiliations

  1. Department of Agronomy and USDA-ARS-FCR, Iowa State University, 50011, Ames, IA, USA

    Vladimir Kanazin & Randy C. Shoemaker

  2. Plant, Soil and Environmental Science Department, Montana State University, 59717, Bozeman, MT, USA

    Tom Blake

Authors
  1. Vladimir Kanazin
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  2. Tom Blake
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  3. Randy C. Shoemaker
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Communicated by H. Saedler

Journal paper No. J-16127 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa

Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the United States Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable

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Kanazin, V., Blake, T. & Shoemaker, R.C. Organization of the histone H3 genes in soybean, barley and wheat. Molec. Gen. Genet. 250, 137–147 (1996). https://doi.org/10.1007/BF02174172

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  • DOI: https://doi.org/10.1007/BF02174172

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