Abstract
TheMagnaporthe grisea repeat (MGR) sequence MGR586 has been widely used for population studies of the rice blast fungus, and has enabled classification of the fungal population into hundreds of genetic lineages. While studying the distribution of MGR586 sequences in strains ofM. grisea, we discovered that the plasmid probe pCB586 contains a significant amount of single-copy DNA. To define precisely the boundary of the repetitive DNA in pCB586, this plasmid and four cosmid clones containing MGR586 were sequenced. Only 740 bp of one end of the 2.6-bp insert in the pCB586 plasmid was common to all clones. DNA sequence analysis of cosmid DNA revealed that all the cosmids contained common sequences beyond the cloning site in pCB586, indicating that the repetitive DNA in the fingerprinting clone is part of a larger element. The entire repetitive element was sequenced and found to resemble an inverted repeat transposon. This putative transposon is 1.86 kb in length and has perfect terminal repeats of 42 bp, which themselves contain direct repeats of 16 bp. The internal region of the transposon possesses one open reading frame which shows similarity at the peptide level to the Pot2 transposon fromM. grisea and Fot1 fromFusarium oxysporum. Hybridization studies using the entire element as a probe revealed that some strains ofM. grisea, whose DNA hybridized to the pCB586 probe, entirely lacked MGR586 transposon sequences.
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Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Borromeo ES, Nelson RJ, Bonman JM, Leung H (1993) Genetic differentiation among isolates ofPyricularia infecting rice and weed hosts. Phytopathology 83:393–399
Correa-Victoria FJ, Zeigler RS (1993) Pathogenic variability inPyricularia grisea at a “hot spot” breeding site in Eastern Colombia. Plant Disease 77:1029–1035
Correa-Victoria FJ, Zeigler RS, Levy M (1994) Virulence characteristics of genetic families ofPyricularia grisea in Colombia. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. CAB International, Wallingford, UK, pp 211–229
Daboussi M-J, Langin T, Brygoo Y (1992) Fot1, a new family of fungal transposable elements. Mol Gen Genet 232:12–16
Dobinson KF, Harris RE, Hamer JE (1993)Grasshopper, a long terminal repeat (LTR) retroelement in the phytopathogenic fungusMagnaporthe grisea. Mol Plant-Microbe Interact 6:114–126
Farman ML, Tosa Y, Nitta N and Leong SA (1996) MAGGY, a retrotransposon in the genome of the rice blast fungusMagnaporthe grisea. Mol Gen Genet (Previous paper)
Hamer JE, Givan S (1990) Genetic mapping with dispersed repeated sequences in the rice blast fungus: mapping theSMO locus. Mol Gen Genet 223:487–495
Hamer JE, Farrall L, Orbach MJ, Valent B, Chumley FG (1989) Host species-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen. Proc Natl Acad Sci USA 86:9981–9985
Kachroo PK, Chattoo BB, Leong SA (1994) Pot2, an inverted repeat transposon fromMagnaporthe grisea. Mol Gen Genet 245:339–348
Kolmer J, Ellingboe AH (1988) Genetic relationships between fertility and pathogenicity and virulence to rice inMagnaporthe grisea. Can J Bot 66:891–897
Langin T, Capy P, Daboussi M-J (1995) The transposable elementimpala, a fungal member of theTc1-mariner superfamily. Mol Gen Genet 246:19–28
Lebrun MH, Capy MP, Garcia N, Duterte M, Brygoo Y, Nottheghem JL, Valés M (1990) Biology and genetics ofPyricularia oryzae andPyricularia grisea populations: current situation and development of RFLP markers. In: Banta SJ, Argosino GS (eds) Second International Rice Genetics Symposium. International Rice Research Institute, Los Baños, Philippines
Leong SA, Farman ML, Smith J, Budde A, Tosa Y, Nitta N (1994) Molecular-genetic approach to the study of cultivar specificity in the rice blast fungus. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. CAB International Wallingford, UK, pp 87–110
Leung H, Williams PH (1985) Genetic analysis of electrophoretic enzyme variants, mating type, and hermaphroditism inPyricularia oryzae Cavara. Can J Genet Cytol 27:697–704
Leung H, Borromeo ES, Bernado MA, Notteghem JL (1988) Genetic analysis of virulence in the rice blast fungusMagnaporthe grisea. Phytopathology 78:1227–1233
Levy M, Romao J, Marchetti MA, Hamer JE (1991) DNA finger-printing with a dispersed repeated sequence resolves pathotype diversity in the rice blast fungus. Plant Cell 3:95–102
Levy M, Correa-Victoria FJ, Zeigler RS, Xu S, Hamer JE (1993) Genetic diversity of the rice blast fungus in a disease nursery in Colombia. Phytopathology 83:1427–1433
Mei B, Budde, AD, Leong SA (1993)sid1, a gene initiating siderophore biosynthesis inUstilago maydis: molecular characterization, regulation by iron and role in phytopathogenicity. Proc Natl Acad Sci USA 90:903–907
Moerman DG, Waterston RH (1989) Mobile elements inCaenorhabditis elegans and other nematodes. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington DC, pp 537–556
Romao J, Hamer JE (1992) Genetic organization of a repeated DNA sequence family in the rice blast fungus. Proc Natl Acad Sci USA 89:5316–5320
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Shull V, Hamer JE (1994) Genomic structure and variability inPyricularia grisea. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. CAB International, Wallingford, UK, pp 65–86
Valent B, Chumley FG (1991) Molecular genetic analysis of the rice blast fungus,Magnaporthe grisea. Annu Rev Phytopathol 29:443–467
Valent B, Chumley FG (1994) Avirulence genes and mechanisms of genetic instability in the rice blast fungus. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. CAB International, Wallingford, UK, pp 111–153
Valent B, Farrall L, Chumley FG (1991)Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. Genetics 127:87–101
Xia JQ, Correll JC, Lee FN, Marchetti MA, Rhoads DD (1993) DNA fingerprinting to examine microgeographic variation in theMagnaporthe grisea (Pyricularia grisea) population in two rice fields in Arkansas. Phytopathology 83:1029–1035
Zeigler RS, Cuoc LX, Scott RP, Bernado MA, Chen DH, Valent B, Nelson RJ (1995) The relationship between lineage and virulence inPyricularia grisea in the Philippines. Phytopathology 85:443–451
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Farman, M.L., Taura, S., Leong, S.A. et al. TheMagnaporthe grisea DNA fingerprinting probe MGR586 contains the 3′ end of an inverted repeat transposon. Molec. Gen. Genet. 251, 675–681 (1996). https://doi.org/10.1007/BF02174116
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DOI: https://doi.org/10.1007/BF02174116