Perspectives in Drug Discovery and Design

, Volume 5, Issue 1, pp 225–233 | Cite as

Suramin: A polysulfonated compound that inhibits the binding of HIV-1 gp120 to GalCer/sulfatide and blocks the CD4-independent pathway of HIV-1 infection in mucosal epithelial cells

  • Djilali Hammache
  • Olivier Delézay
  • Jacques Fantini
  • Nouara Yahi
Part VII. Inhibition Of HIV Entry Into CD4− Cells

Summary

Infection of human colonic epithelial HT-29 cells by human immunodeficiency virus type 1 (HIV-1) occurs independently of CD4, the main HIV-1 receptor expressed on lymphocytes and macrophages. Recent studies by our group have shown that HT-29 cells express the glycosphingolipid galactosylceramide (GalCer), a potential alternative receptor for the HIV-1 envelope glycoprotein gp120. The binding of recombinant gp120 to GalCer is blocked by monoclonal antibodies directed against the third variable region (V3) of gp120, suggesting that the V3 domain is implicated in GalCer recognition. The interaction between GalCer and the V3 loop of gp120 is specifically inhibited by suramin, a polysulfonated naphthylurea previously characterized as an inhibitor of retroviral reverse transcriptases. The biochemical and virological data presented in this report show that suramin is a competitive inhibitor of the GalCer-dependent pathway of HIV-1 infection in HT-29 cells.

Keywords

Suramin Mucosal Epithelial Cell Envelope Glycoprotein Gp120 Recombinant Gp120 Naphthylurea 

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Copyright information

© ESCOM Science Publishers B.V 1996

Authors and Affiliations

  • Djilali Hammache
    • 1
  • Olivier Delézay
    • 1
  • Jacques Fantini
    • 1
  • Nouara Yahi
    • 1
  1. 1.Faculté de Médecine NordCNRS URA 1455, Institut Fédératif Jean RocheMarseille Cédex 20France

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