Abstract
Attempts to characterize thegrpD55 mutation ofEscherichia coli have led us to conclude that the gene had been assigned an incorrect map position. The mutation was found to cotransduce withmalF3089:: Tn10 (at ∼91.5 min) and adnaB-expressing plasmid was able to complement fully thegrpD55 defect in λ replication. These studies strongly suggest thatgrpD55 is an allele ofdnaB and is localized near 92 min on theE. coli linkage map.
References
Arai K, Yasuda S, Kornberg A (1981) Mechanism ofdnaB protein action. J Biol Chem 256:5247–5252
Bachmann BJ (1990) Linkage map ofEscherichia coli K-12, edition 8. Microbiol Rev 54:130–197
Bezanson GS, Iyer N (1975a)dnaB gene ofEscherichia coli K-12 affects superinfection inhibition between F' plasmids. J Bacteriol 123:137–146
Bezanson GS, Iyer N (1975b) Restoration of F' superinfection inhibition in a DnaB mutant ofEscherichia coli upon construction of heterozygous DnaB merodiploids of P1 lysogens carrying adnaB analogue. J Bacteriol 123:147–153
Bull HJ (1995) Bacteriophage lambda replication-coupled processes: genetic elements and regulatory choices. Ph.D. thesis, University of Saskatchewan, Saskatoon, Canada
Ippen-Ihler K, Skurray RA (1993) Genetic organization of transferrelated determinants on the sex factor F and related plasmids, In: D. B. Clewell (ed) Bacterial Conjugation. Plenum Press, New York, pp 23–52
Johnson RC, Ball CA, Pfeffer D, Simon MI (1988) Isolation of the gene encoding the Hin recombinational enhancer binding protein. Proc Natl Acad Sci USA 85:3484–3488
Kohara Y, Aklyama K, Isono K (1987) The physical map of the wholeE. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508
LeBowitz JH, McMacken R (1986) TheEscherichia coli dnaB replication protein is a DNA helicase. J Biol Chem 261:4738–4748
LeBowitz JH, Zylicz M, Georgopoulos C, McMacken R (1985) Initiation of DNA replication on single-stranded DNA templates catalyzed by purified replication proteins of bacteriophage λ andEscherichia coli. Proc Natl Acad Sci USA 82:3988–3992
McMacken R, Mensa-Wilmot K, Alfano C, Seaby R, Carrol K, Gomes B, Stephens K (1988) Reconstitution of purified protein systems for the initiation and regulation of bacteriophage λ DNA replication. Cancer Cells 6:25–34
Miller JH (1992) A short course in bacterial genetics: a laboratory manual and handbook forEscherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Nakayama N, Arai N, Bond M, Kaziro Y, Arai K (1984) Nucleotide sequence ofdnaB and the primary structure of thednaB protein fromEscherichia coli. J Biol Chem 259:97–101
Ninnemann O, Koch C, Kahmann R (1992) TheE. coli fis promoter is subject to stringent control and autoregulation. EMBO J 11:1075–1083
Novick RP (1969) Extrachromosomal inheritance in bacteria. Bacteriol Rev 33:210–235
Saito H, Uchida H (1977) Initiation of the DNA replication of bacteriophage lambda inEscherichia coli K12. J Mol Biol 113:1–25
Saito H, Uchida H (1978) Organization and expression of thednaJ anddnaK genes ofEscherichia coli K12. Mol Gen Genet 164:1–8
Singer M, Baker TA, Schnitzler G, Deischel SM, Dove W, Jaacks KJ, Grossman AD, Erickson JW, Gross CA (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping ofEscherichia coli. Microbiol Rev 53:1–24
Willetts NS (1972) The genetics of transmissible plasmids. Annu Rev Genet 6:257–268
Wymann C, Vasilikiotis C, Ang D, Georgopoulos C, Echols H (1993) Function of the GrpE heat shock protein in bidirectional unwinding and replication from the origin of phage λ. J Biol Chem 268:25192–25196
Yochem J, Uchida H, Sunshine M, Saito H, Georgopoulos CP, Feiss M (1978) Genetic analysis of two genes,dnaJ anddnaK, necessary forEscherichia coli and bacteriophage lambda DNA replication. Mol Gen Genet 164:9–14
Zylicz M, LeBowitz JH, McMacken R, Georgopoulos C (1983) The dnaK protein ofEscherichia coli possesses an ATPase and autophosphorylating activity and is essential in an in vitro DNA replication system. Proc Natl Acad Sci USA 80:6431–6435
Zylicz M, Yamamoto T, Mckittrick N, Sell S, Georgopoulos C (1985) Purification and properties of the dnaJ replication protein ofEscherichia coli. J Biol Chem 260: 7591–7598
Zylicz M, Ang D, Georgopoulos C (1987) The grpE protein ofEscherichia coli. J Biol Chem 262:17437–17442
Zylicz M, Ang D, Liberek K, Georgopoulos C (1989) Initiation of λ DNA replication with purified host- and bacteriophage-encoded proteins: the role of the dnaK, dnaJ and grpE heat shock proteins. EMBO J 8:1601–1608
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Communicated by B. J. Kilbey
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Bull, H.J., Hayes, S. ThegrpD55 locus ofEscherichia coli appears to be an allele ofdnaB . Molec. Gen. Genet. 252, 755–760 (1996). https://doi.org/10.1007/BF02173984
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DOI: https://doi.org/10.1007/BF02173984