Abstract
The tomato geneLeRse-1 was isolated from a root hair-specific cDNA library. The amino acid sequence of theLeRse-1 product displayed a high degree of identity to that ofAgMtd, a mannitol dehydrogenase-encoding gene from celery. Expression ofLeRse-1 was found to be restricted to the root organs of tomato, with no detectable transcripts in stems and leaves. Transcripts ofLeRse-1 were detected in root hairs, as well as other root tissues of mature plants and seedlings. Root-specificLeRse-1 expression increased upon exposure of the shoot to light. Since roots were kept in darkness, shoot-to-root communication is required for light-inducedLeRse-1 RNA accumulation.
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References
Altschul SF, Gish W, Miller W, Meyers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Baucher M, van Doorsselaere J, Gielen J, van Montagu M, Inzé D, Boerjan W (1995) Genomic nucleotide sequence of anArabidopsis thaliana gene encoding a cinnamyl alcohol dehydrogenase. Plant Physiol 107:285–286
Kiedrowski S, Kawalleck P, Hahlbrock K, Somssich IE, Dangl JL (1992) Rapid activation of a novel plant defense gene is strictly dependent on theArabidopsis RPM1 disease resistance locus. EMBO J 11:4684–4689
Lauter F-R, Ninnemann O, Buchner M, Riesmeyer J, Frommer WB (1996) Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato. Proc Natl Acad Sci USA 93:8139–8144
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual (2nd edn) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Smart DR, Bloom AJ (1993) Relationships between the kinetics of NH +4 and NO −3 absorption and growth in the cultivated tomato. Plant Cell and Environment 16:259–267
Stoop JMH, Pharr DM (1992) Partial purification and characterization of mannitol: mannose-1-oxidoreductase from celeriac (Apium graveolens var.rapaceum) roots. Arch Biochem Biophys 298:612–619
Stoop JMH, Williamson JD, Conkling MA, Pharr DM (1995) Plant Physiol 108:1219–1225
Williamson JD, Stoop JMH, Massel MO, Conkling MA, Pharr DM (1995) Sequence analysis of a mannitol dehydrogenase cDNA from plants reveals a function for a pathogenesis-related protein ELI3. Proc Natl Acad Sci USA 92:7148–7152
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Communicated by H. Saedler
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Lauter, F.R. Root-specific expression of theLeRse-1 gene in tomato is induced by exposure of the shoot to light. Molec. Gen. Genet. 252, 751–754 (1996). https://doi.org/10.1007/BF02173983
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DOI: https://doi.org/10.1007/BF02173983