Molecular and General Genetics MGG

, Volume 251, Issue 5, pp 526–531 | Cite as

Telomere-homologous sequences occur near the centromeres of many tomato chromosomes

  • G. G. Presting
  • A. Frary
  • K. Pillen
  • S. D. Tanksley
  • G. G. Presting
Original Paper

Abstract

Several bacteriophage lambda clones containinginterstitialtelomererepeats (ITR) were isolated from a library of tomato genomic DNA by plaque hybridization with the clonedArabidopsis thaliana telomere repeat. Restriction fragments lacking highly repetitive DNA were identified and used as probes to map 14 of the 20 lambda clones. All of these markers mapped near the centromere on eight of the twelve tomato chromosomes. The exact centromere location of chromosomes 7 and 9 has recently been determined, and all ITR clones that localize to these two chromosomes map to the marker clusters known to contain the centromere. High-resolution mapping of one of these markers showed cosegregation of the telomere repeat with the marker cluster closest to the centromere in over 9000 meiotic products. We propose that the map location of interstitial telomere clones may reflect specific sequence interchanges between telomeric and centromeric regions and may provide an expedient means of localizing centromere positions.

Key words

High-resolution mapping Karyotype evolution Restriction fragment length polymorphism (RFLP) Zycopersicon 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • G. G. Presting
    • 1
  • A. Frary
    • 1
  • K. Pillen
    • 1
  • S. D. Tanksley
    • 1
  • G. G. Presting
    • 2
  1. 1.Department of Plant Breeding and BiometryCornell UniversityIthacaUSA
  2. 2.Plant Science CenterCornell UniversityIthacaUSA

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