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Blood-retinal barrier permeability to carboxyfluorescein and fluorescein in monkeys

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Abstract

Lipid solubility is a major determinant of permeability across the blood-brain barrier, to which the blood-retinal barrier (BRB) has many similarities. Carboxyfluorescein is a dye with about 1/1000 the lipid solubility of fluorescein, but their molecular sizes and spectral characteristics are similar. We studied the importance of lipid solubility in BRB permeability by comparing the BRB permeabilities to these two dyes. Dye in the vitreous and plasma of four monkeys was measured by fluorophotometry. The estimated inward permeability coefficients (P in) were 11 ± 7.4 × 10−6) cm/min (mean and SD) for carboxyfluorescein and 21 ± 5.9 × 10−6 cm/min for fluorescein. The ratio of the means was 1/1.9, far from the expected 1/1000. This finding suggests that the BRB does not function as a continuous lipid membrane and that other factors are more important determinants of permeability for these dyes than lipid solubility.

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References

  • Araie M, Sawa M, Nagataki S, Mishima S (1980) Aqueous humor dynamics in man as studied by oral fluorescein. Jpn J Ophthalmol 24:346–362

    Google Scholar 

  • Blair NP, Zeimer RC, Rusin MM, Cunha-Vaz JG (1983) Outward transport of fluorescein from the vitreous in normal human subjects. Arch Ophthalmol 101:1117–1121

    Google Scholar 

  • Blair NP, Jones CW, Rusin MM (1984) Pathophysiology of the blood-retinal barrier in experimental diabetes: vitreous fluorophotometry using carboxyfluorescein and fluorescein. Arch Ophthalmol 102:1810–1814

    Google Scholar 

  • Blair NP, Rusin MM, Shakin E (1985) The effect of pH on the transfer of fluorescein across the blood-retinal barrier. Invest Ophthalmol Vis Sci 26:1133–1139

    Google Scholar 

  • Cunha-Vaz JG (1980) Sites and functions of the blood-retinal barriers. In: Cunha-Vaz JG (ed) The blood-retinal barriers. Plenum Press, New York, pp 101–117

    Google Scholar 

  • Cunha-Vaz JG, Maurice DM (1967) The active transport of fluorescein by the retinal vessels and the retina. J Physiol 191:467–486

    Google Scholar 

  • Grimes PA, Stone RA, Laties AM, Li W (1982) Carboxyfluorescein: a probe of the blood-ocular barriers with lower membrane permeability than fluorescein. Arch Ophthalmol 100: 635–639

    Google Scholar 

  • Kirber WM, Nichols CW, Grimes PA, Winegrad AI, Laties AM (1980) A permeability defect of the retinal pigment epithelium: occurrence in early streptozocin diabetes. Arch Ophthalmol 98:725–728

    Google Scholar 

  • Laties AM, Rapoport SI (1976) The blood-ocular barriers under osmotic stress: studies on the freeze-dried eye. Arch Ophthalmol 94:1086–1091

    Google Scholar 

  • Rapoport SI (1976a) Sites and functions of the blood-brain barrier. In: Rapoport SI (ed) Blood-brain barrier in physiology and medicine. Raven Press, New York, pp 43–86

    Google Scholar 

  • Rapoport SI (1976b) Regulation of drug entry into the nervous system. In: Rapoport SI (ed) Blood-brain barrier in physiology and medicine. Raven Press, New York, pp 153–176

    Google Scholar 

  • Rapoport SI, Fredericks WR, Laties AM (1980) Reversible opening of the retinal pigment epithelium by hypercapnia. Exp Eye Res 30:129–141

    Google Scholar 

  • Shinowara NL, Grimes PA, Rapoport SI, Laties AM (1983) Acidosis alters fluorescein permeability: differential tracer penetration through pigment epithelium. Arch Ophthalmol 101:1771–1774

    Google Scholar 

  • Stein WD (1981) Permeability for lipophilic molecules. In: Bonting SL, de Pont JJHHM (eds) Membrane transport. Elsevier/North-Holland Biomedical Press, New York, pp 1–28

    Google Scholar 

  • Tsuboi S, Fujimoto T, Uchihori Y, Emi K, Iizuka S, Kishida K, Manabe R (1984) Measurement of retinal permeability to sodium fluorescein in vitro. Invest Ophthalmol Vis Sci 25:1146–1150

    Google Scholar 

  • Zeimer RC, Blair NP, Cunha-Vaz JG (1983a) Vitreous fluorophotometry for clinical research. I. Description and evaluation of a new fluorophotometer. Arch Ophthalmol 101:1753–1756

    Google Scholar 

  • Zeimer RC, Blair NP, Cunha-Vaz JG (1983b) Vitreous fluorophotometry for clinical research. II. Method of data acquisition and processing. Arch Ophthalmol 101:1757–1761

    Google Scholar 

  • Zeimer RC, Blair NPP, Cunha-Vaz JG (1983c) Pharmacokinetic interpretation of vitreous fluorophotometry. Invest Ophthalmol Vis Sci 24:1374–1381

    Google Scholar 

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Authors and Affiliations

  1. Department of Ophthalmology, College of Medicine, University of Illinois at Chicago, USA

    Norman P. Blair & Mark M. Rusin

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  1. Norman P. Blair
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  2. Mark M. Rusin
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This investigation was supported in part by Public Health Service Grants EY03227 and EY03106 (Dr. Blair) and Ophthalmic Research Center Core Grant EY1792 from the National Eye Institute, Bethesda, Maryland, USA

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Blair, N.P., Rusin, M.M. Blood-retinal barrier permeability to carboxyfluorescein and fluorescein in monkeys. Graefe's Arch Clin Exp Ophthalmol 224, 419–422 (1986). https://doi.org/10.1007/BF02173356

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  • DOI: https://doi.org/10.1007/BF02173356

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