Short-term organ culture of the retinal pigment epithelium in microtitration plates: Ultrastructural studies

  • N. M. McKechnie
  • W. A. Keegan
  • C. A. Converse
  • W. S. Foulds


Microtitration plates were used to culture simultaneously multiple, small (6 mm diameter) explants of bovine retinal pigment epithelium (RPE). Evaluation of tissue by light microscopy and by scanning and transmission electron microscopy after various incubation periods up to 6 h showed that RPE maintained in this system retains near normal morphology. Initially, the explanted RPE lacks apical microvilli, but during the first 2–3 h in culture recovery of apical microvilli occurs. The results suggest that the system is suitable for short-term maintenance of RPE for experimental purposes. Moreover, the ability to culture up to 16 explants from one bovine eye aids statistical evaluation of RPE behaviour under varying experimental conditions.


Public Health Microscopy Electron Microscopy Transmission Electron Microscopy Light Microscopy 
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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • N. M. McKechnie
    • 1
  • W. A. Keegan
    • 2
  • C. A. Converse
    • 2
  • W. S. Foulds
    • 1
  1. 1.Department of OphthalmologyUniversity of GlasgowScotland
  2. 2.Department of PharmacyUniversity of StrathclydeGlasgowScotland

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