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Fibrinolysis in cornea and conjunctiva: Evidence of two types of activators

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Abstract

The nature of corneal and conjunctival plasminogen activators (PAs) from human and rabbit eyes was examined in tissue culture. The fibrinolytic activity of culture fluid from human corneas was low, roughly one-eighth of that of rabbits. The main activity was found to be of the urokinase (UK) type, as demonstrated by crossed immunoelectrophoresis against alpha2-antiplasmin after incubation with mixed plasma. The fibrinolytic activity of culture fluid from human conjunctival tissue was higher and a mixed secretion of tissue type (tPA) and UK activator was demonstrated. These activators were separated by affinity chromatography against Sepharose-immobilized antibodies against tPA. Fibrinolytic activity in tears was quenched by antibodies against tPA but not by the use of anti-UK antibodies. Thus, in man, tear fibrinolytic activity may depend primarily on a release of PAs from conjunctival tissue.

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Author notes

  1. Maurizio Pandolfi

    Present address: Department of Ophthalmology, King Fahd Hospital of the University, P.O. Box 2208, Al-Khobar, Saudi Arabia

Authors and Affiliations

  1. Department of Ophthalmology and Coagulation Laboratory, University Hospital, Malmö, Sweden

    Esbjörn Lantz & Maurizio Pandolfi

Authors
  1. Esbjörn Lantz
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  2. Maurizio Pandolfi
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Lantz, E., Pandolfi, M. Fibrinolysis in cornea and conjunctiva: Evidence of two types of activators. Graefe's Arch Clin Exp Ophthalmol 224, 393–396 (1986). https://doi.org/10.1007/BF02173351

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  • DOI: https://doi.org/10.1007/BF02173351

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