Abstract
The recessive nuclear mutationiojap (ij) in maize produces striped plants with normal chloroplasts in green sectors and poorly developed chloroplasts in the white sectors. Theij mutation is also characterized by an array of additional phenotypic affects which suggests a pivotal role forIj in chloroplast development. TheIj gene from maize has been isolated; however, the sequence has not provided information on the action of theIj gene product. As an extension of the analysis of theIj gene we have generated a number of independent transposon insertion mutations using the Robertson'sMutator (Mu). Insertions were found to be distributed throughout the 5′ half of the gene and all showed a similar striping pattern to that of the originalij mutation,ij-ref. While several of the new mutant alleles producedij-homologous transcripts none produced detectable levels of the Ij polypeptide product. Chloroplast development in some cells appears to be able to proceed in the absence of the Ij polypeptide.
References
Barker RF, Thompson DV, Talbot DR, Swanson J, Bennetzen L (1984) Nucleotide sequence of the maize transposable elementMu1. Nucleic Acids Res 12:5955–5967
Bennetzen JL, Springer PS, Cresse AD, Hendrickx M (1993) Specificity and regulation of theMutator transposable element system in maize. Crit Rev Plant Sci 12:57–95
Chomezynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
Chomet P, Lisch D, Hardeman KJ, Chandler VL, Freeling M (1991) Identification of a regulatory transposon that controls theMutator transposable element system in maize. Genetics 129:261–270
Coe EH, Thompson DL, Walbot V (1982) Nuclear genes and chloroplast modifications in maize. Stadler Genet Symp 14:29–46
Coe EH, Thompson DL, Walbot V (1988) Phenotypes mediated by theiojap genotype in maize. Amer J Bot 75:634–644
Freeling M (1988) Mutagenesis using Robertson'smutator lines and consequent insertions at theAdh1 gene in maize. In: Nelson O (ed) Plant transposable elements. Plenum Press, New York, pp 279–291
Hake S (1992) Unraveling the knots in plant development. Trends Genet 8:109–114
Han C-d, Coe EH, Martienssen RA (1992) Molecular cloning and characterization ofiojap (ij), pattern striping gene of maize. EMBO J 11:4037–4046
Han C-d, Patrie W, Polacco, M, Coe EH (1993) Aberrations in plastid transcripts and deficiency of plastid DNA in striped and albino mutants in maize. Planta 191:552–563
Hershberger RJ, Warren CA, Walbot V (1991) Mutator activity in maize correlates with the presence and expression of theMu transposable elementMu9. Proc Natl Acad Sci USA 88:10198–10202
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Martienssen RA, Barkan A, Freeling M, Taylor WC (1989) Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson'sMutator. EMBO J 81:1633–1639
Qin M, Robertson DS, Ellingboe AH (1991) Cloning of theMutator transposable elementMuA2, a putative regulator of somatic mutability of theal-Mum2 allele in maize. Genetics 129:845–854
Rhoades MM (1943) Genic induction of an inherited cytoplasmic difference. Proc Natl Acad Sci USA 29:327–329
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Shumway LK, Weier TE (1967) The chloroplast structure ofiojap maize. Amer J Bot 54:773–780
Thompson D, Walbot V, Coe EH (1983) Plastid development iniojap- andchloroplast mutator- affected maize plants. Amer J Bot 70:940–950
Walbot V, Coe EH (1979) Nuclear geneiojap conditions a programmed change to ribosome-less plastids inZea mays. Proc Natl Acad Sci USA 76:2760–2764
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Communicated by R. G. Herrmann
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Byrne, M., Taylor, W.C. Analysis ofMutator-induced mutations in theIojap gene of maize. Molec. Gen. Genet. 252, 216–220 (1996). https://doi.org/10.1007/BF02173223
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DOI: https://doi.org/10.1007/BF02173223