Advertisement

Molecular and General Genetics MGG

, Volume 252, Issue 1–2, pp 155–161 | Cite as

Chromosomal heteromorphism linked to the mating type locus of the oomycetePhytophthora infestans

  • H. S. Judelson
Original Paper

Abstract

The mating type locus of the oomycete,Phytophthora infestans, is embedded in a region of DNA that displays distorted and non-Mendelian segregation. By using DNA probes linked to the mating type locus to genetically and physically characterize that region, a large zone of chromosomal heteromorphism was detected. LocusS1 was shown to represent a tandemly repeated array of DNA that was typically present in a hemizygous state in A1 isolates while being absent from A2 isolates. The analysis of the parents and progeny of seven crosses indicated that the tandem array was linked in cis to the A1-determining allele of the mating type locus. A worldwide survey of genotypically diverse field isolates ofP. infestans indicated thatS1 was present in each of 48 isolates of the A1 mating type that were tested, but was absent in 46 of 47 A2 strains. Physical analysis ofS1 indicated that the tandemly repeated DNA sequence spanned about 300 kb and had evolved from a 1.35-kb monomer. Internal deletions occurred withinS1 during sexual propagation. This and other mutations apparently contributed to a high degree of polymorphism within theS1 array.

Key words

Potato late blight Population genetics Sex chromosome Tandem repeat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Caten CE, Jinks JL (1968) Spontaneous variability of isolates ofPhytophthora infestans. I. Cultural variation. Can J Bot 46:329–348Google Scholar
  2. Chu G, Vollrath D, Davis RW (1986) Separation of large DNA molecules by contour-clamped homogeneous electric field. Science 234:1582–1585Google Scholar
  3. Doshi P, Kaushal S, Benyajati C, Wu CI (1991) Molecular analysis of the responder satellite DNA inDrosophila melanogaster: DNA bending, nucleosome structure, and Rsp-binding proteins. Mol Biol Evol 8: 721–741Google Scholar
  4. Ferris PJ, Goodenough UW (1994) The mating-type locus ofChlamydomonas reinhardtii contains highly rearranged DNA sequences. Cell 76:1135–1145Google Scholar
  5. Förster H, Coffey MD (1990) Mating behavior ofPhytophthora parasitica: evidence for sexual recombination in oospores using DNA restriction fragment length polymorphisms as genetic markers. Exp Mycol 14:351–359Google Scholar
  6. Fry WE, Goodwin SB, Matuszak JM, Spielman LJ, Milgroom MG (1992) Population genetics and intercontinental migrations ofPhytophthora infestans. Annu Rev Phytopath 60:107–129Google Scholar
  7. Gallegly ME (1968) Genetics of pathogenicity ofPhytophthora infestans. Annu Rev Phytopath 6: 75–396Google Scholar
  8. Graves JA, Watson JM (1991) Mammalian sex chromosomes: evolution of organization and function. Chromosoma 101:63–68Google Scholar
  9. Judelson HS (1993) Efficient cotransformation mediated by intermolecular ligation occurring in vivo in the oomycetePhytophthora infestans. Mol Gen Genet 239:241–250Google Scholar
  10. Judelson HS, Spielman LJ, Shattock RC (1995) Genetic mapping and non-Mendelian segregation of mating type loci in the oomycetePhytophthora infestans. Genetics 141:503–512Google Scholar
  11. Lyttle TW (1991) Segregation distorters. Annu Rev Genet 25:511–557Google Scholar
  12. Mead LJ, Gillespie MT, Irving LB, Campbell LJ (1994) Homozygous and hemizygous deletions of 9p centromeric to the interferon genes in lung cancer. Cancer Res 52:2307–2309Google Scholar
  13. Morrow B, Goldberg R, Carlson C, Das Gupta R, Sirotkin H, Collins J, Dunham I, O'Donnell H, Scambler P, Shprintzen R (1995) Molecular definition of the 22q11 deletions in velocardio-facial syndrome. Am J Hum Genet 56:1391–1403Google Scholar
  14. Nowak R (1994) Mining treasures from ‘junk DNA’. Science 263:608–610Google Scholar
  15. Ott J (1985) Analysis of human genetic linkage. Johns Hopkins University Press, BaltimoreGoogle Scholar
  16. Pukkila PJ, Skrzynia C (1993) Frequent changes in the number of reiterated ribosomal RNA genes throughout the life cycle of the basidiomyceteCoprinus cinereus. Genetics 133:203–211Google Scholar
  17. Schlötterer C, Tautz D (1994) Chromosomal homogenity ofDrosophila ribosomal DNA arrays suggests intrachromosomal exchanges drive concerted evolution. Curr Biol 4:777–783Google Scholar
  18. Shattock RC, Tooley PW, Fry WE (1986) Genetics ofPhytophthora infestans: determination of recombination, segregation, and selfing by isozyme analysis. Phytopathology 76:410–413Google Scholar
  19. Shaw DS (1988) ThePhytophthora species. In: Sidhu GS (ed) Advances in Plant Pathology, vol 6. Academic Press, New York, pp 27–51Google Scholar
  20. Spielman LJ, McMaster BJ, Fry WE (1989) Dominance and recessiveness at loci for virulence against potato and tomato inPhytophthora infestans. Theor Appl Genet 77:832–838Google Scholar
  21. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1991) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • H. S. Judelson
    • 1
  1. 1.Department of Plant PathologyUniversity of CaliforniaRiversideUSA

Personalised recommendations