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Sexual Plant Reproduction

, Volume 9, Issue 4, pp 209–215 | Cite as

Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperature

  • A. TouraevEmail author
  • A. Indrianto
  • I. Wratschko
  • O. Vicente
  • E. Heberle-Bors
Original Paper

Abstract

We have established an efficient method to induce embryo formation from isolated wheat (Triticum aestivum L.) microspores. Culture of excised anthers under starvation and heat shock conditions induced the formation of embryogenic microspores at high frequency in nine Austrian winter wheat genotypes, including cultivars that had been considered as recalcitrant in anther culture. Percoll gradient centrifugation of the mechanically isolated microspores allowed us to obtain homogeneous populations of embryogenic microspores in all genotypes which, after transfer to a rich medium containing immature ovaries for conditioning, divided and produced globular embryos. Thousands of embryos were produced in one petri dish. Many of these embryos developed into plantlets after transfer to a solid medium without ovaries.

Key words

Triticum aestivum L. Microspore embryogenesis Starvation Heat shock Haploid production 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • A. Touraev
    • 1
    Email author
  • A. Indrianto
    • 1
  • I. Wratschko
    • 1
  • O. Vicente
    • 1
  • E. Heberle-Bors
    • 1
  1. 1.Institute of Microbiology and GeneticsVienna BiocenterViennaAustria

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