Abstract
Two genes fromThermoanaerobacterium thermosulfurigenes EM1 were identified which are predicted to encode a xylanase (XynA) and a polygalacturonate hydrolase (PglA). ThexynA gene has the potential to encode a 1234-amino acid product consisting of a signal peptide followed by a repeated domain, a xylanase family F domain, two cellulose-binding domains and a triplicated sequence at its C-terminus. The genepglA is predicted to encode a product of 1148 amino acids consisting of a signal sequence followed by a fibronectin type III-like domain (Fn3 domain), the catalytic domain, a Gly/Thr/Ser/Asn-rich segment and a triplicated domain. The triplicated segments at the C-termini of deduced XynA and PglA are about 95% identical to each other and to the S-layer-like domains of the previously characterized pullulanase (AmyB) from the same organism. In contrast, sequence comparisons revealed only distant amino acid sequence similarities between the fibronectin type III-like domains of PglA and AmyB fromT. thermosulfurigenes EM1.
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References
Antranikian G, Herzberg C, Mayer F, Gottschalk G (1987) Changes in the cell envelope structure ofClostridium sp. strain EM1 during massive production of α-amylase and pullulanase. FEMS Microbiol Lett 41:193–197
He SY, Collmer A (1990) Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-α-D-galacturonosidase-encodingpehX gene ofErwinia chrysanthemi EC16. J Bacteriol 172:4988–4995
Henrissat B, Bairoch A (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293:781–788
Jespersen HM, MacGregor EA, Sierks MR, Svensson B (1991) Comparison of the domain level organization of starch hydrolases and related enzymes. Biochem J 280:51–55
Lee Y-E, Lowe SE, Henrissat B, Zeikus JG (1993) Characterization of the active site and thermostability regions of endoxylanase fromThermoanaerobacterium saccharolyticum B6A-RI. J Bacteriol 175:5890–5898
Lemaire M, Ohayon H, Gounon P, Fujino T, Béguin P (1995) OlpB, a new outer layer protein ofClostridium thermocellum, and binding of its S-layer-like domains to components of the cell envelope. J Bacteriol 177:2451–2459
Little E, Bork P, Doolittle RF (1994) Tracing the spread of fibronectin type III domains in bacterial glycohydrolases. J Mol Evol 39:631–643
Lupas A, Engelhardt H, Peters J, Santarius U, Volker S, Baumeister W (1994) Domain structure of theAcetogenium kivui surface layer revealed by electron crystallography and sequence analysis. J Bacteriol 176:1224–1233
Madi E, Antranikian G (1989) Identification of a starch degrading anaerobic thermophile producing thermostable α-amylase and pullulanase. Appl Microbiol Biotechnol 30:422–425
Matuschek M, Burchhardt G, Sahm K, Bahl H (1994) Pullulanase ofThermoanaerobacterium thermosulfurigenes EM1 (Clostridium thermosulfurogenes): molecular analysis of the gene, composite structure of the enzyme, and a common model for its attachment to the cell surface. J Bacteriol 176:3295–3302
Melasniemi H, Paloheimo M, Hemiö L (1990) Nucleotide sequence of the α-amylase-pullulanase gene fromClostridium thermohydrosulfuricum. J Gen Microbiol 136:447–454
Saarilahti HT (1993) Characterization of polygalacturonases. Gene 124:145–147
Shine J, Dalgarno L (1974) The 3′-terminal sequence ofEscherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome-binding sites. Proc Natl Acad Sci USA 71:1342–1346
Von Heijne G (1988) Transcending the impenetrable: how proteins come to terms with membranes. Biochim Biophys Acta 947:307–333
Winterhalter C, Heinrich P, Candussio A, Wich G, Liebl W (1995) Identification of a novel cellulose-binding domain within the multidomain 120 kDa xylanase XynA of the hyperthermophilic bacteriumThermotoga maritima. Mol Microbiol 15:431–444
Zhang J-X, Martin J, Flint HJ (1994) Identification of non-catalytic conserved regions in xylanases encoded by thexynB andxynD genes of the cellulolytic rumen anaerobeRuminococcus flavefaciens. Mol Gen Genet 245:260–264
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Matuschek, M., Sahm, K., Zibat, A. et al. Characterization of genes fromThermoanaerobacterium thermosulfurigenes EM1 that encode two glycosyl hydrolases with conserved S-layer-like domains. Molec. Gen. Genet. 252, 493–496 (1996). https://doi.org/10.1007/BF02173016
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DOI: https://doi.org/10.1007/BF02173016