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The synthesis of theStreptomyces reticuli cellulase (Avicelase) is regulated by both activation and repression mechanisms

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Abstract

TheStreptomyces reticuli cellulase (Cell, Avicelase) hydrolyzes crystalline cellulose (Avicel) efficiently to cellobiose. The synthesis of the enzyme is induced by Avicel and repressed by glucose. DNA-binding proteins were purified from inducedS. reticuli mycelia by affinity chromatography using the upstream region of thecell gene linked to Sepharose. The enriched protein(s) provoked a gel electrophoresis mobility shift of the upstream region, irrespective of the presence or absence of a 14-bp palindromic sequence, and enhanced the transcription of thecell gene by theS. reticuli RNA polymerase in vitro. The binding site (GTGACTGAGCGCCG) for the protein(s) was located in the vicinity of a DNA bend upstream of the transcriptional start site. Results of physiological studies, deletion and gel-shift analyses lead to the conclusion that a 14-bp palindrome (TGGGAGCGCTCCCA) — situated between the transcriptional start site and the structure gene — is the operator for a repressor protein. The data presented suggest that the two identified cis-acting elements, in cooperation with an activator and a repressor, mediate regulation ofcell transcription.

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  1. FB Biologie/Chemie, Universität Osnabrück, Barbarastrasse 11, 49069, Osnabrück, Germany

    S. Walter & H. Schrempf

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  1. S. Walter
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  2. H. Schrempf
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Communicated by H. Böhme

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Walter, S., Schrempf, H. The synthesis of theStreptomyces reticuli cellulase (Avicelase) is regulated by both activation and repression mechanisms. Molec. Gen. Genet. 251, 186–195 (1996). https://doi.org/10.1007/BF02172917

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  • DOI: https://doi.org/10.1007/BF02172917

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