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The glucose repressor genecre1 ofTrichoderma: Isolation and expression of a full-length and a truncated mutant form

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Abstract

Thecre1 genes of the filamentous fungiTrichoderma reesei andT. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor genecreA ofAspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. Thecre1 promoters contain several sequence elements that are identical to the previously identified binding sites forA. nidulans CREA. Steady-state mRNA levels forcre1 of theT. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest thatcre1 expression may be autoregulated. TheT. reesei strain Rut-C30, a hyperproducer of cellulolytic enzymes, was found to express a truncated form of thecre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-lengthcre1 gene into this strain caused glucose repression ofcbh1 expression, demonstrating thatcre1 regulates cellulase expression.

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  1. C. Thrane

    Present address: Department of Plant Biology, Plant Pathology Section, Royal Veterinary and Agricultural University, Copenhagen, Denmark

Authors and Affiliations

  1. VTT Biotechnology and Food Research, P.O. Box 1500, FIN-02044 VTT, Espoo, Finland

    M. Ilmén, C. Thrane & M. Penttilä

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  1. M. Ilmén
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  2. C. Thrane
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  3. M. Penttilä
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Communicated by C. A. M. J. J. van den Hondel

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Ilmén, M., Thrane, C. & Penttilä, M. The glucose repressor genecre1 ofTrichoderma: Isolation and expression of a full-length and a truncated mutant form. Molec. Gen. Genet. 251, 451–460 (1996). https://doi.org/10.1007/BF02172374

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  • DOI: https://doi.org/10.1007/BF02172374

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