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Biotherapy

, Volume 2, Issue 1, pp 1–12 | Cite as

Enhancement of depressed lymphokine activated killer cell activity in patients with hepatocellular carcinoma

  • Toshiji Saibara
  • Saburo Onishi
  • Yasushi Matsuura
  • Masanao Fujikawa
  • Hiroshi Sakaeda
  • Youichi Matsunaga
  • Yasutake Yamamoto
Article

Abstract

Hepatocellular carcinoma (HCC) patients can be divided into two groups according to the degree of lymphokine activated killer (LAK) cell activity; a high LAK activity group (H-LAK-HCC) and a low LAK activity group (L-LAK-HCC). Interferon-gamma (IFN-gamma) production is severely defective in L-LAK-HCC but not defective in H-LAH-HCC. IFN-gamma production is suppressed with the addition of anti-Tac in dose dependent manner, though LAK activity is suppressed only in the presence of high concentration of anti-Tac. LAK activity is suppressed with the addition of anti-IFN-gamma, which is most prominent when the antibody is present during the first 12 hr of incubation. LAK generation is enhanced with the addition of recombinant IFN-gamma, which is most prominent when it is present during the first 12 hr of incubation. However, this enhancing effect is less prominent in L-LAK-HCC as compared to normals, liver cirrhosis, and H-LAK-HCC. This enhancement is regarded to depend on the presence of Leu7+ and Leu11+ subset, as this enhancement is abandoned and IFN-gamma production is inhibited when either of these subsets is deleted. These data suggest that IFN-gamma production and the participation of Leu7+ and Leu11+ subsets is important in sufficient LAK generation, and that poor IFN-gamma production and insufficient response to IFN-gamma may be the cause of severely defective LAK generation in L-LAK-HCC.

Key words

hepatocellular carcinoma gamma-interferon interleukin 2 lymphokine activated killer cell 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Toshiji Saibara
    • 1
  • Saburo Onishi
    • 1
  • Yasushi Matsuura
    • 1
  • Masanao Fujikawa
    • 1
  • Hiroshi Sakaeda
    • 1
  • Youichi Matsunaga
    • 1
  • Yasutake Yamamoto
    • 1
  1. 1.First Department of MedicineKochi Medical SchoolKochiJapan

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