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Inhibition of growth of rat hepatoma by local injection of liposomes containing recombinant interleukin-2

Antitumor effect of IL-2 liposome

  • Published:
Biotherapy

Abstract

Human recombinant interleukin-2 (IL-2) was entrapped in liposome, consisting of egg phosphatidylcholine (PC) and cholesterol.

The peri-tumor injections of IL-2 liposome inhibited significantly the growth of solid tumor and prolonged the survival time of rats with solid tumors which were induced by a subcutaneous (s.c.) inoculation of AH-66 cells.

Immunohistochemical staining of peritoneal exudate cells and tumor tissues revealed a marked accumulation of activated macrophages in and around the tumor tissues induced by the local injections of IL-2 liposome.

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Abbreviations

egg PC:

egg phosphatidylcholine

IL-2:

interleukin 2

IL-2 liposome:

IL-2 entrapped in liposomes

i.p.:

intraperitoneal

i.v.:

intravenous

JU:

Jurkat unit

LAK:

lymphokine activated killer

mAb:

monoclonal antibody

PBS:

phosphate buffered saline solution

PEC:

peritoneal exudate cells

saline liposome:

saline entrapped in liposomes

s.c.:

subcutaneous

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Authors and Affiliations

  1. Second Department of Surgery, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan

    Hiroyuki Konno M.D. & Shukichi Sakaguchi

  2. Second Department of Anatomy, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-31, Hamamatsu, Japan

    Akira Yamashita

  3. Department of Microbiology, School of Medicine, Keio University, 35 Shinano-machi, Shinjuku-ku, 160, Tokyo, Japan

    Takushi Tadakuma

Authors
  1. Hiroyuki Konno M.D.
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  2. Akira Yamashita
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  3. Takushi Tadakuma
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  4. Shukichi Sakaguchi
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Konno, H., Yamashita, A., Tadakuma, T. et al. Inhibition of growth of rat hepatoma by local injection of liposomes containing recombinant interleukin-2. Biotherapy 3, 211–218 (1991). https://doi.org/10.1007/BF02171684

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  • DOI: https://doi.org/10.1007/BF02171684

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