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Unusual antiproliferative effects of transforming growth factors-β 1 andβ 2 against primary cells from human tumors

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Biotherapy

Abstract

Transforming growth factorsβ 1 andβ2 (TGF-β1 andβ2), tested in a clonogenic assay against primary cells from human tumors, suppress proliferation to different extents. In nineteen of twenty-six cell cultures, proliferation was < 50% of control with factor at 0.04 or 0.4 nM. Of these, TGF-β 2 was more active than TGF-β1 in fourteen; and TGF-β1 was more active than TGF-β2 in five. In seven of the nineteen, proliferation was 0% with one or the other factor. In contrast, cisplatin was much less effective in inhibiting proliferation of some of the same cells even at 1,000 or more times the molar concentration of the factors. Surprisingly, when TGF-β 1 and TGF-β2 were combined at equal concentrations, the antiproliferative effect of one was cancelled or markedly inhibited by the other.

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Author notes

  1. John M. McPherson

    Present address: Integrated Genetics, 31 New York Avenue, 01701, Framingham, MA, USA

Authors and Affiliations

  1. Target Therapeutics, 130 Rio Robles, 95134, San Jose, CA, USA

    Anna Marie Daniels & John R. Daniels

  2. Celtrix Laboratories, Collagen Corporation, 2500 Faber Place, 94303, Palo Alto, CA, USA

    John M. McPherson & Karl A. Piez

Authors
  1. Anna Marie Daniels
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  2. John M. McPherson
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  3. John R. Daniels
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  4. Karl A. Piez
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Daniels, A.M., McPherson, J.M., Daniels, J.R. et al. Unusual antiproliferative effects of transforming growth factors-β 1 andβ 2 against primary cells from human tumors. Biotherapy 1, 133–137 (1989). https://doi.org/10.1007/BF02170882

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  • DOI: https://doi.org/10.1007/BF02170882

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