Summary
The oxygen uptake of two strains ofMycelium Radicis atrovirens was measured volumetrically in the respirometer apparatus ofvon Euler, Myrbäck andNilsson. The vessels employed were 100 cm3 Erlenmeyer flasks with center cup containing the solution of potassium hydroxide used for absorption of carbon dioxide. The mycelium was grown in a submerged state in shaking cultures according to the method ofWikén andSomm. The oxygen consumption of the growing young mycelium in a glucose-ammonium tartrate medium amounted to about 2 × 10−2 cm3 per milligram of dry weight per hour. The respiratory activity of the washed mycelium in distilled water or phosphate buffer was equal to that shown by the intact mycelium in its actual culture medium. The high autorespiration (endogenous respiration) thus observed was reduced appreciably by starvation for 48 hours in phosphate buffer under aerated conditions. The autorespiration of the starved mycelium amounted to 0.1−0.3×10−2 cm3 per milligram of dry weight per hour, whereas its oxygen uptake in solutions of glucose, succinate, fumarate, and citrate was equal to 0.6−1.7×10−2 cm3.
Literatur
A. J. Kluyver undL. H. C. Perquin, Biochem. Z.266, 68, 82 (1933).
L. H. C. Perquin,Bijdrage tot de kennis der oxydatieve dissimilatie van Aspergillus niger van Tieghem (Diss. Delft 1938).
S. C. Prescott undC. G. Dunn,Industrial Microbiology (Mc Graw-Hill Book Co., New York 1940).
J. W. Foster,Chemical Activities of Fungi (Academic Press Inc., New York 1949).
K. Bernhauer undJ. Rauch, Biochem. Z.319, 77, 102 (1948).
T. Wikén, H. G. Keller, C. L. Schelling undA. Stöckli, Exper.7, 237 (1951).
T. Wikén undH. Somm, Exper.8, 110 (1952).
T. Wikén, H. G. Keller, C. L. Schelling undA. Stöckli, Exper.7, 237 (1951).
C. L. Schelling,Zur Kenntnis der Physiologie von Mycelium Radicis atrovirens Melin mit besonderer Berücksichtigung der Verwertbarkeit verschiedener Kohlenstoffquellen (Diss. ETH., Zürich 1952).
T. Wikén undH. Somm, Exper.8, 110 (1952).
R. Nilsson,Die Methoden der Fermentforschung (hg. vonBamann undMyrbäck)3, 2150 (1941).
J. W. Foster,Chemical Activities of Fungi, (New York 1949).
B. S. Gould undA. A. Tytell, J. Gen. Physiol.24, 655 (1941).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wikén, T., Somm, H. Atmungsversuche mit submersem Schüttelmyzel des WurzelpilzesMycelium Radicis atrovirens in der Apparatur nach von Euler, Myrbäck und Nilsson. Experientia 8, 140–142 (1952). https://doi.org/10.1007/BF02170219
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02170219