Journal of Radioanalytical and Nuclear Chemistry

, Volume 166, Issue 5, pp 431–440 | Cite as

Bacterial biosorption and retention of thorium and uranyl cations by Mycobacterium smegmatis

  • Y. Andres
  • H. J. MacCordick
  • J. -C. Hubert


Biosorption of Th4+ and UO 2 2+ ions, both separately and in mixed equimolar ratio, was carried out using nitrate-buffered solutions of the cations at pH 1 in the presence of 5%w/w non-proliferative cell suspensions of Mycobacterium smegmatis. At equilibrium following a 3 h treatment, specific adsorption for 2 mM Th and U was, respectively, 102 and 115 μmol g−1 dry biomass for individual solutions and 102 and 42 μmol g−1 for the mixed 2/2 mM solution. Desorption studies of the cation-loaded biomass preparations in aqueous media and in soilbacterial suspensions within the pH range <1 to 11 showed that leaching of throium was generally less than 1% at pH 1–11 after 7 d, whereas uranium was leached to the extent of 2% at pH 1 and up to 10% under the same conditions in Th−U mixtures.


Biomass Uranium Cell Suspension Leaching Aqueous Medium 
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  1. 1.
    G.M. Gadd,Experientia, 46 (1990) 834.Google Scholar
  2. 2.
    M.D. Mullen et al.,Appl. Environ. Microbiol., 55 (1989) 3143.PubMedGoogle Scholar
  3. 3.
    S.E. Shumate, G.W. Strandberg, in Comprehensive Biotechnology (Ed. by M. Moo-Young, C.N. Robinson, J.A. Howell) Pergamon Press, New York, 0985, Vol. 4, p. 235.Google Scholar
  4. 4.
    B.E. Stout, K.M. Schaab, Contribution No 120, 204th ACS National Meeting, Washington DC, August 23–28, 1992.Google Scholar
  5. 5.
    H.J. MacCordick, J.M. Paulus, M.G. Bontems,J. Radioanal. Nucl. Chem. Lett., 145 (1990) 231.Google Scholar
  6. 6.
    H.J. MacCordick, J.-C. Hubert, J.J. Schleiffer,J. Radioanal. Nucl. Chem. Lett., 135 (1989) 349.Google Scholar
  7. 7.
    A.M. Marqués et al.,Appl. Microbiol. Biotechnol., 35 (1991) 406.Google Scholar
  8. 8.
    G.W. Strandberg, S.E. Shumate, J.R. Parrott, Jr.,Appl. Environ. Microbiol., 41 (1981) 237.Google Scholar
  9. 9.
    M. Tsezos, B. Volesky,Biotechnol. Bioeng., 23 (1981) 583.Google Scholar

Copyright information

© Akadémiai Kiadó 1992

Authors and Affiliations

  • Y. Andres
    • 1
  • H. J. MacCordick
    • 2
  • J. -C. Hubert
    • 1
  1. 1.Laboratoire de Microbiologie, URA CNRS 1481, Institut Le BelUniversité Louis PasteurStrasbourgFrance
  2. 2.Laboratoire de Chimie NucléaireCentre de Recherches Nucléaires et Université Louis PasteurStrasbourgFrance

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