Journal of Radioanalytical and Nuclear Chemistry

, Volume 155, Issue 3, pp 155–168 | Cite as

Determination of thyroxine and 3,5,3′-triiodothyronine by sub- and super-equivalence isotope dilution analysis

  • J. Lesný
  • Z. Koreňová
  • J. Klas
Article

Abstract

Trace amounts of thyroxine in model samples (160.0 ng.ml−1 and 20.0 ng.ml−1), and thyroxine and 3,5,3′-triiodothyronine in blood plasma were determined by sub- superequivalence isotope dilution analysis and radio-immunoassay technique. Hormones were labelled by125I. The separation of antibody-bound hormone from free hormone was performed by ultracentrifugation. The results show higher accuracy of the sub- super-equivalence isotope dilution method over that of radioimunoassay.

Keywords

Physical Chemistry Inorganic Chemistry Trace Amount Blood Plasma Thyroxine 

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Copyright information

© Akadémiai Kiadó 1991

Authors and Affiliations

  • J. Lesný
    • 1
  • Z. Koreňová
    • 2
  • J. Klas
    • 3
  1. 1.Department of Environmental ScienceSlovak Technical UniversityBratislavaCzechoslovakia
  2. 2.Water Research InstituteBratislavaCzechoslovakia
  3. 3.Central Laboratory of Chemical TechniquesSlovak Technical UniversityBratislavaCzechoslovakia

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