Abstract
Trace amounts of thyroxine in model samples (160.0 ng.ml−1 and 20.0 ng.ml−1), and thyroxine and 3,5,3′-triiodothyronine in blood plasma were determined by sub- superequivalence isotope dilution analysis and radio-immunoassay technique. Hormones were labelled by125I. The separation of antibody-bound hormone from free hormone was performed by ultracentrifugation. The results show higher accuracy of the sub- super-equivalence isotope dilution method over that of radioimunoassay.
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Lesný, J., Koreňová, Z. & Klas, J. Determination of thyroxine and 3,5,3′-triiodothyronine by sub- and super-equivalence isotope dilution analysis. Journal of Radioanalytical and Nuclear Chemistry Letters 155, 155–168 (1991). https://doi.org/10.1007/BF02166640
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DOI: https://doi.org/10.1007/BF02166640