Journal of Radioanalytical and Nuclear Chemistry

, Volume 155, Issue 3, pp 155–168 | Cite as

Determination of thyroxine and 3,5,3′-triiodothyronine by sub- and super-equivalence isotope dilution analysis

  • J. Lesný
  • Z. Koreňová
  • J. Klas


Trace amounts of thyroxine in model samples (160.0−1 and 20.0−1), and thyroxine and 3,5,3′-triiodothyronine in blood plasma were determined by sub- superequivalence isotope dilution analysis and radio-immunoassay technique. Hormones were labelled by125I. The separation of antibody-bound hormone from free hormone was performed by ultracentrifugation. The results show higher accuracy of the sub- super-equivalence isotope dilution method over that of radioimunoassay.


Physical Chemistry Inorganic Chemistry Trace Amount Blood Plasma Thyroxine 
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  1. 1.
    S.A. Berson, R.S. Yalow,Nature (London), 184 (1959) 1648.Google Scholar
  2. 2.
    B.M. Jaffe, H.R. Behrmen, Methods of Hormone Radioimmunoassay, Academic Press, New York, 1974.Google Scholar
  3. 3.
    H. Brener, D. Hamel, H.L. Krüskemper, Methoden der Hormonebestimmung, G. Thieme, Stuttgart, 1975.Google Scholar
  4. 4.
    G.E. Abraham, Handbook of Radioimmunoassay, M. Dekker, New York, 1977.Google Scholar
  5. 5.
    T.S. Work, E. Work, An Introduction to Radioimmunoassay and Related Techniques, 2nd. Ed. Elsevier, Amsterdam, 1982.Google Scholar
  6. 6.
    R.Y. Anders,Chimia, 37 (1983) 257.Google Scholar
  7. 7.
    J. Klas, J. Tölgyessy, J. Lesný, Sub- super-equivalent Isotope Dilution Analysis, Veda, Bratislava, 1985.Google Scholar
  8. 8.
    J. Lesný, J. Tölgyessy, L. Čulák, J. Klas, B. Novruzi, A. Cela,J. Radioanal. Nucl. Chem., Letters, 135 (1989) 9.Google Scholar
  9. 9.
    J. Klas, J. Tölgyessy, E.H. Klehr,Radiochem. Radioanal. Lett., 18 (1974) 83.Google Scholar
  10. 10.
    J.C. Miller, J.N. Miller, Statistics for Analytical Chemistry, John Wiley & Sons, New York, 1984.Google Scholar
  11. 11.
    K. Eckschlager,Chem. listy, 68 (1974) 750.Google Scholar

Copyright information

© Akadémiai Kiadó 1991

Authors and Affiliations

  • J. Lesný
    • 1
  • Z. Koreňová
    • 2
  • J. Klas
    • 3
  1. 1.Department of Environmental ScienceSlovak Technical UniversityBratislavaCzechoslovakia
  2. 2.Water Research InstituteBratislavaCzechoslovakia
  3. 3.Central Laboratory of Chemical TechniquesSlovak Technical UniversityBratislavaCzechoslovakia

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