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Immunoreactivity of125I-papain labelled by different methods

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Abstract

Three different methods of papain iodination/with chloramine-T, lactoperoxidase and conjugation with Bolton-Hunter reagent/were compared. The highest yield of125I-papain was obtained using lactoperoxidase which enables to achieve the highest immunoreactivity.125I-papain, labelled in this manner, is suitable for the radioimmunoassay of papain.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28, Prague 6, Czechoslovakia

    P. Rauch, L. Fukal & J. Káš

  2. Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10, Prague 6, Czechoslovakia

    R. Tykva

Authors
  1. P. Rauch
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  2. L. Fukal
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  3. J. Káš
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  4. R. Tykva
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Rauch, P., Fukal, L., Káš, J. et al. Immunoreactivity of125I-papain labelled by different methods. Journal of Radioanalytical and Nuclear Chemistry Letters 85, 115–121 (1984). https://doi.org/10.1007/BF02166523

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  • DOI: https://doi.org/10.1007/BF02166523

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