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Some interferences in radioimmunoassay of aflatoxin B1

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Abstract

The specificity of radioimmunoassay of aflatoxin B1 was tested. Relative cross-reactivity of used antiserum with aflatoxins B1, B2, G1, G2 and M1 was found to be 100%, 24%, 44.2%, 10.3%, and 1.4%, respectively. The interference of coumarin, albumins, steroids and ethylvanilin was estimated also in radioimmunoassay of aflatoxin B1. Thus these compounds may cause a false positive finding of aflatoxin B1.

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Authors and Affiliations

  1. Department of Biological Sciences, Agriculture University, 165 00, Prague 6, Czechoslovakia

    L. Fukal & Z. Sova

  2. Department of Veterinary Medicine, Agriculture University, 165 00, Prague 6, Czechoslovakia

    H. Reisnerová

Authors
  1. L. Fukal
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  2. Z. Sova
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  3. H. Reisnerová
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Fukal, L., Sova, Z. & Reisnerová, H. Some interferences in radioimmunoassay of aflatoxin B1 . Journal of Radioanalytical and Nuclear Chemistry Letters 108, 297–303 (1987). https://doi.org/10.1007/BF02165188

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  • DOI: https://doi.org/10.1007/BF02165188

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