Abstract
The feasibility of utilizing liquid scintillation counting as a detection method for the liquid chromatographic analysis of99Tc is evaluated. The chemical quenching of constituents commonly found in liquid chromatographic mobile phases is investigated, and quench corrections are presented. Quantification of99Tc is achieved over a concentration range of 10−3 to 10−8M on quiescent KTCO4 solutions, with sample volumes of 0.3 ml, yielding a counting efficiency from 51 to 59% for a narrow 150 channel window. The potential for the double isotope labelling of technetium complexes and the implications of the liquid scintillation counting of99Tc in flowing eluents are discussed.
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References
J. K. Kaye, N. E. Ballou,Anal. Chem., 50 /1978/ 2076.
C. R. Walker, B. W. Short, H. S. Short, in Radioelement analysis: Progress and Problems, /W. S. Lyon, Ed./, Ann Arbor Science Press, Ann Arbor, Michigan, 1980, pp. 101–110.
T. L. Rucker, W. R. Mullins, in Radioelement Analysis: Progress and Problems /W. S. Lyon, Ed./, Ann Arbor Science Press, Ann Arbor, Michigan, 1980, pp. 95–100.
R. A. Pacer,Int. J. Appl. Radiation Isotopes, 31 /1980/ 731.
T. C. Pinkerton, W. R. Heineman, E. Deutsch,Anal. Chem., 52 /1980/ 1106.
T. C. Pinkerton, D. L. Ferguson, E. Deutsch, W. R. Heineman, K. Libson,Int. J. Appl. Radiation Isotopes, 33 /1982/ 907.
S. Tanabe, J. P. Zodda, K. Libson, E. Deutsch, W. R. Heineman,Int. J. Appl. Radiation Isotopes, 34 /1983/ 1585.
J. A. G. M. Wandenbrand, H. A. Das, B. G. Dekker, C. L. Deligny, C. J. A. Vandenhamer,Int. J. Appl. Radiation Isotopes, 33 /1982/ 917.
J. P. Zodda, W. R. Heineman, T. W. Gilbert, E. Deutsch,J. Chrom. Biomed. Appl., 227 /1982/ 249.
M. D. Loberg, A. T Fields,Int. J. P. Appl. Radiation Isotopes, 29 /1978/ 167.
S. C. Srivastava, G. E. Meinken, P. Richards, L. A. Ford, W. R. Benson, Third World Congress on Nuclear Medicine and Biology, Paris, France, August 29, 1982.
J. W. Rzeszotarski,Chrom. Sci. Ser., 10 /1979/ 475.
T. F. Kellogg,Anal. Biochem., 134 /1983/ 137.
C. T. Peng in The Current Status of Liquid Scintillation Counting, /E. D. Bransome Jr., Ed./, Grune and Stratton, Publ., New York, N.Y., 1970, p. 283.
D. Freifelder, in Physical Biochemistry, W. H. Freeman and Co., San Francisco, CA, 1976, 88–120.
G. A. Bray,Anal. Biochem., 1 /1960/ 279.
E. Schram, R. Lombaert, in Organic Scintillation Detectors, Elsevier Publ. Co., 1963, p. 21.
D. Hoch, T. C. Pinkerton, Purdue University, West Lafayette, Indiana/unpublished results/.
L. R. Snyder, J. J. Kirkland, in Introduction to Modern Liquid Chromatography, U. Wiley and Sons, Inc., New York, N.Y., 1979, p. 127.
Technical Notes, Vol. 1, Part 1, Radiomatic Instrument and Chemical Co., Inc., Tampa, FL 1981.
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Lawson, B.L., Powell, C.R. & Pinkerton, T.C. Quantification of99Tc by liquid scintillation counting: An evaluation of chemical quenching by liquid chromatographic eluents. Journal of Radioanalytical and Nuclear Chemistry Letters 94, 71–86 (1985). https://doi.org/10.1007/BF02164605
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DOI: https://doi.org/10.1007/BF02164605