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A rapid radioimmunoassay procedure for thyroxin

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Abstract

A sensitive thyroxin /T4/ radioimmunoassay procedure with a short incubation time /10 min/ similar to a conventional stat test in clinical chemistry is described. The assay parameters such as concentration of antisera and125I-T4, and incubation temperature that affect the kinetics of antigen-antibody reaction have been studied. Within- and between-assay variations were less than 7% CV. Analysis of 45 serum samples by the proposed method and by a conventional assay gave similar results /Y=1.05X–0.187; r=0.990/.

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References

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Authors and Affiliations

  1. Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Trombay, 400 085, Bombay, India

    R. S. Mani

  2. Regional Centre for Radiopharmaceuticals, Bhabha Atomic Research Centre, KMIO Campus, 560 029, Bangalore, India

    V. Bhupal

Authors
  1. V. Bhupal
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  2. R. S. Mani
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Bhupal, V., Mani, R.S. A rapid radioimmunoassay procedure for thyroxin. Journal of Radioanalytical and Nuclear Chemistry Letters 107, 371–376 (1986). https://doi.org/10.1007/BF02164202

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  • DOI: https://doi.org/10.1007/BF02164202

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