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Radioiodination and quality control of human thyrotropin for radioimmunoassay

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Abstract

This work reports the radioiodination of human thyrotropin (hTSH) in our laboratory (IPEN) and evaluates its quality in comparison with a commercial product. The radioiodination yield obtained in 20 experiments ranged from 18.5 to 56.3%, while the purification recovery ranged from 75.5 to 124.0% and the specific activity ranged from 1.01 to 3.10 MBq μg−1. The values for the distribution coefficient revealed in the purification of radioiodinated hTSH ranged from 0.232 to 0.371. When tested concomitantly in the same radioimmunoassay system, the IPEN and the commercial tracer presented parallel standard curves. A highly significant correlation ion was observed between the quality control samples estimated through both curves (p<0.001). These results confirm the quality of the hTSH radioiodinated at IPEN and suggest the acquirement of self-sufficiency in this “in vitro” nuclear technology.

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Authors and Affiliations

  1. Laboratory of Medical Investigation (LIM-25), Faculty of Medicine of São Paulo University, Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP), São Paulo, Brazil

    V. C. Borghi, L. H. Lin, S. R. Silva, E. P. Haber & B. L. Wajchenberg

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  1. V. C. Borghi
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  2. L. H. Lin
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  3. S. R. Silva
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  4. E. P. Haber
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  5. B. L. Wajchenberg
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Borghi, V.C., Lin, L.H., Silva, S.R. et al. Radioiodination and quality control of human thyrotropin for radioimmunoassay. Journal of Radioanalytical and Nuclear Chemistry Letters 200, 337–349 (1995). https://doi.org/10.1007/BF02163787

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  • DOI: https://doi.org/10.1007/BF02163787

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