Abstract
Rat luteinizing hormone /LH/ was labelled with125I by the Chloramine T method.125I-LH, used as tracer in radioimmunoassay, was separated from the labelling reaction mixture by gel filtration. By using the proper protein/radioiodine ratio in the labelling reaction mixture the specific activity of125I-LH was adjusted to 2.5–20.5 MBq μg−1. The influence of the specific activity on the assay parameters as well as on the tracer stability was investigated.
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Miholics, E., Sárándi, I. & Tóth, G. Effect of the specific activity of the tracer on rat luteinizing hormone radioimmunoassay. Journal of Radioanalytical and Nuclear Chemistry Letters 144, 79–87 (1990). https://doi.org/10.1007/BF02162190
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DOI: https://doi.org/10.1007/BF02162190