Advertisement

Experientia

, Volume 15, Issue 7, pp 278–281 | Cite as

Gallen und Gallensäuren Papierchromatographische Untersuchungen

  • G. L. Szendey
Informationen Studiorum Progressus

Summary

In cases of bile-scretion disturbances substitution-therapy can be performed using suitable animal biles equivalent to human bile. Comparative investigations have been made to establish which animal bile is in practice the most suitable for bile substitutiontherapy based on its bile acid components.

A new paper partition chromatographic method has been applied for separation and identification of free bile acids. Ascending development was used on Schleicher & Schüll 2043b Mgl paper impregnated with 20 v/v% propylene glycol in chloroform. The xylene-methylethylketone 1:1 solvent system gave good separation. The bile acids can be detected by immersing the chromatograms in 20 w/v% SbCl3 in chloroform followed by drying and heating for 5–10 min at 100–110°C. The spots show intense reddish-violet or blue fluorescence in filtered UV-light (see Table 1).

These investigations have shown that human and ox biles are, in contrast to pig bile, physiologically related biological substances regarding their bile acid components.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 2.
    O. Hammarsten, Erg. Physiol.4, 1 (1905).Google Scholar
  2. 3.
    O. Hammarsten,Lehrbuch der physiologischen Chemie (1922), p. 349.Google Scholar
  3. 4.
    B. Isaksson, Acta Soc. med. Upsaliensis59, 307 (1953/54).Google Scholar
  4. 5.
    H. S. Wiggins, Biochem. J.56, XXXIX (1954).Google Scholar
  5. 6.
    I. D. P. Wootton, Biochem. J.53, 85 (1953).PubMedGoogle Scholar
  6. 7.
    J.-C. Encrantz undJ. Sjövall, Acta chem. Scand.34, 1005 (1956).Google Scholar
  7. 8.
    H. Sobotka,Physiological Chemistry of the Bile (Baltimore, London 1937).Google Scholar
  8. 9.
    H. Lettré undH. H. Inhoffen,Über Sterine, Gallensäuren und verwandte Naturstoffe (Stuttgart 1936). Erweiterte Neuausgabe:H. Lettré, H. H. Inhoffen undR. Tschesche (Stuttgart 1954).Google Scholar
  9. 10.
    G. A. D. Haslewood undV. Wootton, Biochem. J.47, 584 (1950).Google Scholar
  10. 11.
    H. Minibeck, Biochem. Z.297, 29 (1938).Google Scholar
  11. 12.
    H. Minibeck, Biochem. Z.297, 40 (1938).Google Scholar
  12. 13.
    Documenta Geigy, 1955, p. 307.Google Scholar
  13. 14.
    B. Flaschenträger,Physiologische Chemie (Berlin-Göttingen-Heidelberg 1956).Google Scholar
  14. 15.
    K. Hinsberg undK. Lang,Medizinische Chemie (München-Berlin-Wien 1957).Google Scholar
  15. 16.
    G. Pause, Fortschr. Med.69, 210 (1951).Google Scholar
  16. 17.
    G. Zeise, Med. Klin.47, 1696 (1952).PubMedGoogle Scholar
  17. 18.
    H. Gross, Therap. Gegenw.95, 423 (1956).Google Scholar
  18. 19.
    Ph. Jacobs, Pharm. Weekblad92, 239 (1957).Google Scholar
  19. 20.
    J. Sjövall, Acta chem. Scand.6, 1552 (1952).Google Scholar
  20. 21.
    J. Sjövall, Acta chem. Scand.8, 339 (1954).Google Scholar
  21. 22.
    J. Sjövall, Ark. Kemi8, 299 (1955).Google Scholar
  22. 23.
    D. Kritschevsky undM. R. Kirk, Arch. Biochem. Biophysics35, 346 (1952).Google Scholar
  23. 24.
    D. Kritschevsky undM. R. Kirk, J. Amer. chem. Soc.74, 4713 (1952).Google Scholar
  24. 25.
    J. Beyreder undH. Rettenbacher-Däubner, Mh. Chem.84, 99 (1953).Google Scholar
  25. 26.
    M. D. Siperstein, F. M. Harold, I. L. Chaikoff undW. G. Dauben, J. biol. Chemistry210, 181 (1954).Google Scholar
  26. 27.
    G. A. D. Haslewood, Biochem. J.56, 581 (1954).PubMedGoogle Scholar
  27. 28.
    I. E. Bush, Biochem. J.50, 370 (1952).PubMedGoogle Scholar
  28. 29.
    G. A. D. Haslewood undJ. Sjövall, Biochem. J.57, 126 (1954).PubMedGoogle Scholar
  29. 30.
    M. Kuroda, J. Jap. biochem. Soc.27, 301 (1955).Google Scholar
  30. 31.
    D. Kritschevsky undR. F. J. McCandless, J. Amer. pharmac. Assoc., Sci. Edit.45, 385 (1956).Google Scholar
  31. 32.
    O. Cerri undA. Spialtini, Bill. chim. pharmac.96, 193 (1957).Google Scholar
  32. 33.
    J. B. Carey Jr. undH. S. Bloch, J. lab. clin. Med.44, 486 (1954).PubMedGoogle Scholar
  33. 34.
    J. B. Carey Jr., Science123 892 (1956).Google Scholar
  34. 35.
    S. Eriksson undJ. Sjövall, Acta chem. Scand.8, 1099 (1954).Google Scholar
  35. 36.
    J. Sjövall, Ark. Kemi8, 317 (1955).Google Scholar
  36. 37.
    F. Bandow, Biochem. Z.301, 37 (1939).Google Scholar
  37. 38.
    L. C. Kier, J. lab. clin. Med.40, 755 (1952).Google Scholar
  38. 39.
    L. C. Kier, J. lab. clin. Med.40, 762 (1952).PubMedGoogle Scholar
  39. 40.
    E. Chabrol, R. Charonnat, J. Cottet undP. Blonde, C. R. Soc. Biol. Filiales115, 834 (1934).Google Scholar
  40. 41.
    Y. Abe, J. Biochem. (Tokyo)25, 181 (1937).Google Scholar
  41. 42.
    E. L. Pratt undH. B. Corbitt, Analyst. Chem.24, 1665 (1952).Google Scholar
  42. 43.
    G. Biglino undM. Gennaro-Soffietti, Ann. Chim. (Roma)41, 603 (1951).Google Scholar
  43. 44.
    F. S. Fowweather, Biochem. J.44, 607 (1949).Google Scholar
  44. 45.
    M. Knedel undK. Neikes, Klin. Wschr.34, 1005 (1956).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1959

Authors and Affiliations

  • G. L. Szendey
    • 1
  1. 1.Forschungslaboratorien der Dr. Schwarz Arzneimittelfabrik GmbH.Monheim bei Düsseldorf

Personalised recommendations