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Partial purification of a plasma-kinin-forming enzyme from horse urine

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Zusammenfassung

Durch Chromatographie mit Diäthylamino-äthyl-cellulose nach Ammoniumsulfat-Fraktionierung wurde ein Plasma-Kinin-freisetzendes Ferment aus Pferdeharn teilweise (80×) gereinigt. Das so gereinigte Präparat war gegen Pferde-, Menschen-, Rinder (Fraktion IV-4)- und Ratten-Blutplasma, nicht aber gegen Schweine-(Fraktion IV-4)-Globuline wirksam. Durch Sojabohnen-Trypsininhibitor oder Ovomucoid erfolgt keine Hemmung. Präparate von verschiedenem Reinheitsgrad zeigten entsprechendep-Toluenesulfonyl-l-arginin-methylester-spaltende und Kinin-freisetzende Wirksamkeit.

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Authors and Affiliations

  1. Laboratórios de Farmacologia e Bioquímica, Escola Paulista de Medicina, São Paulo, Brasil

    Eline S. Prado & J. L. Prado

Authors
  1. Eline S. Prado
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  2. J. L. Prado
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Additional information

Read before the XIIth Annual Meeting of the Sociedade Brasileira para o Progresso da Ciência, Piracicaba, July 1960; supported by Grant No. 58217 from the Rockefeller Foundation.

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Prado, E.S., Prado, J.L. Partial purification of a plasma-kinin-forming enzyme from horse urine. Experientia 17, 31–32 (1961). https://doi.org/10.1007/BF02157937

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