Summary
For the isolation of chloroplasts in non-aqueous media the following organic solvents are suitable: pentane, hexane, heptane, petroleum ether and carbon tetrachloride. These solvents cause a minimum loss of lipids.
The loss of lipids and some lipid components of frozendried and ground shoots fromElodea canadensis during the isolation of chloroplasts in non-aqueous media (petroleum ether b. r. 60–80°C and petroleum ether-carbon tetrachloride mixtures) is reported.
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Thalacker, R., Behrens, M. Zur Wahl der organischen Lösungsmittel bei der Gewinnung von Chloroplasten in nichtwässerigem Milieu. Experientia 16, 165–167 (1960). https://doi.org/10.1007/BF02157739
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DOI: https://doi.org/10.1007/BF02157739