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Zur Wahl der organischen Lösungsmittel bei der Gewinnung von Chloroplasten in nichtwässerigem Milieu

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Summary

For the isolation of chloroplasts in non-aqueous media the following organic solvents are suitable: pentane, hexane, heptane, petroleum ether and carbon tetrachloride. These solvents cause a minimum loss of lipids.

The loss of lipids and some lipid components of frozendried and ground shoots fromElodea canadensis during the isolation of chloroplasts in non-aqueous media (petroleum ether b. r. 60–80°C and petroleum ether-carbon tetrachloride mixtures) is reported.

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References

  1. M. Behrens undR. Thalacker, Naturwissenschaften44, 621 (1957).

    Article  Google Scholar 

  2. U. Heber, Ber. dtsch. bot. Ges.70, 371 (1957).

    CAS  Google Scholar 

  3. C. R. Stocking, Plant Physiol.34, 56 (1959).

    Article  CAS  Google Scholar 

  4. R. Thalacker undM. Behrens, Z. Naturf.14b, 443 (1959).

    Article  CAS  Google Scholar 

  5. M. Behrens, Hoppe-Seylers Z.209, 59 (1932).

    Article  CAS  Google Scholar 

  6. M. Behrens, Hoppe-Seylers Z.232, 263 (1935).

    Article  CAS  Google Scholar 

  7. R. Feulgen, M. Behrens undS. Mahdihassan, Hoppe-Seylers Z.246, 203 (1937).

    Article  CAS  Google Scholar 

  8. M. Behrens, Hoppe-Seylers Z.253, 185 (1938).

    Article  CAS  Google Scholar 

  9. A. L. Dounce, C. H. Tishkoff, S. R. Barnett undR. M. Freer, J. gen. Physiol.33, 629 (1950).

    Article  CAS  Google Scholar 

  10. H. Stern undA. E. Mirsky, J. gen. Physiol.36, 181 (1952).

    Article  CAS  Google Scholar 

  11. V. Allfrey, H. Stern, A. E. Mirsky undH. Saetren, J. gen. Physiol.35, 529 (1952).

    Article  CAS  Google Scholar 

  12. H. Stern undA. E. Mirsky, J. gen. Physiol.37, 177 (1954).

    Article  Google Scholar 

  13. E. R. M. Kay, R. M. S. Smellie, G. E. Humphrey undJ. N. Davidson, Biochem. J.62, 160 (1956).

    Article  CAS  Google Scholar 

  14. A. E. Beet, Nature175, 514 (1955).

    Article  Google Scholar 

  15. K. Lohmann undL. Jendrassik, Biochem. Z.178, 419 (1926).

    CAS  Google Scholar 

  16. D. I. Arnon, Plant Physiol.24, 1 (1949).

    Article  CAS  Google Scholar 

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Thalacker, R., Behrens, M. Zur Wahl der organischen Lösungsmittel bei der Gewinnung von Chloroplasten in nichtwässerigem Milieu. Experientia 16, 165–167 (1960). https://doi.org/10.1007/BF02157739

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  • DOI: https://doi.org/10.1007/BF02157739

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