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Experientia

, Volume 16, Issue 6, pp 286–287 | Cite as

Zur Methodik der Gefrierschnitt-Mikroautoradiographie

  • Erna Reinholz
  • V. Belloch-Zimmermann
  • Christl Wirth
Kurze Mitteilungen Pro Experimentis

Summary

A microautoradiographic technique for frozen sections is described. The method makes it possible to follow the distribution of inhaled radon in the organism with high degree of accuracy. After inhaling radon, the mice were killed and stored in liquid air (freezing method ofRajewsky9). The organ sections (5µ thick) were prepared by means of a freezing microtome in the ‘Kryostat’. A thin fixing and protecting layer of chromic alum gelatine was placed between the tissue-sections and the photographic emulsion. The exposure took place in an atmosphere of nitrogen.

References

  1. 1.
    A. Schraub, B. Rajewsky, E. Reinholz, Chr. Wirth undV. Belloch-Zimmermann,Die Verteilung inhalierten Radons im Organismus, Abhandlungen des IX. Int. Congresses für Radiologie (München 1959), im Druck.Google Scholar
  2. 3.
    R. Taugner undU. Wagenmann, Arch. exp. Path. Pharm.234, 336 (1958).Google Scholar
  3. 4.
    G. A. Boyd,Autoradiography in Biology and Medicine (Acad. Press Inc., New York 1955).Google Scholar
  4. 5.
    G. Guidotti undF. Passalacqua, Exper.12, 117 (1956).Google Scholar
  5. 6.
    A. Beiser, Rev. mod. Phys.24, 275 (1952).CrossRefGoogle Scholar
  6. 7.
    G. Albouy undH. Faraggi, J. Phys. Radium10, 105 (1949).Google Scholar
  7. 8.
    W. Lohmann, Z. Naturf.11a, 592 (1956).Google Scholar
  8. 9.
    B. Rajewsky,Naturforschung und Medizin in Deutschland 1939–1946, Bd. 21,Biophysik, Teil I, p. 169.Google Scholar

Copyright information

© Birkhäuser Verlag 1960

Authors and Affiliations

  • Erna Reinholz
    • 1
  • V. Belloch-Zimmermann
    • 1
  • Christl Wirth
    • 1
  1. 1.Max Planck-Institut für BiophysikFrankfurt a. M.Deutschland

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